| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Outline of Final Research Achievements |
A lot of Koshu wine-products contained a proline abundantly. There is indication the proline abundance is one of the factors to reduce the wine quality. Aim of this study is a construction of proline eliminating method from Koshu wine by using yeast. 1) We attempt to construct PRO3 gene disruptant for blocking proline de novo synthesis. However, the yeast strain OC-2 was diploid, it was difficult to construct PRO3 gene double knockout. 2) We found the change lipid composition in expressing PUT4 gene encoded proline transporter in SD medium with 50mM proline. In total lipid composition, palmitic and stearic acid were increased, palmitoleic and oleic acid were decreased adversity. It is unclear the valuable lipid composition of PUT4 gene transformant. 3) We screened a new wild yeast strain which show high proline assimilation. Proline assimilation was tested by proline contained minimum SD medium. As a result, only one strain can assimilate proline over 70%.
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