Three-dimensional super-resolution spectromicroscopy to trace inter-protein interactions and morphological changes in photosynthetic membranes on spatial and temporal domains

2011 Fiscal Year Final Research Report

• Project Area: Molecular Science for Supra Functional Systems ? Development of Advanced Methods for Exploring Elementary Process
• Project/Area Number: 19056012
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Kyoto University
• Principal Investigator: KUMAZAKI Shigeichi 京都大学, 大学院・理学研究科, 准教授 (40293401)
• Project Period (FY): 2007 – 2011
• Keywords: シアノバクテリア / チラコイド膜 / 葉緑体 / 顕微分光 / 光化学系 / 2光子励起 / アンチストークス蛍光 / 近赤外レーザー

Research Abstract

We have found that photosystem I (PSI)-specific fluorescence spectra of thylakoid membranes of cyanobacteria and chloroplasts was very efficiently observed in the case of continuous wave (CW) near infrared (NIR) excitation around 785-820 nm. It was found that the PSI fluorescence in the case of CW NIR excitation is mainly generated by a one-photon excitation mechanism. This phenomenon will be used for studies of PSI/PSII ratio at physiological temperature, since PSI fluorescence has been conventionally observed at low temperatures like 77 K.

Research Products

• [Journal Article] アンチストークス蛍光スペクトルと2光子励起蛍光スペクトルによる光合成膜の顕微分光分析 (2011)
  • Author(s): 熊崎茂一
  • Journal Title: 生物物理 Volume: 51(6) Pages: 274-275
  • DOI: DOI:10.2142/biophys.51.274
• [Journal Article] Anti-Stokes Fluorescence Spectra of Chloroplasts in Parachlorella kessleri and Maize at Room Temperature as Characterized by Near Infrared Continuous Wave Laser Fluorescence Microscopy and Absorption Microscopy (2011)
  • Author(s): Makoto Hasegawa, Takahiko Yoshida, Mitsunori Yabuta, Masahide Terazima, and Shigeichi Kumazaki
  • Journal Title: J. Phys. Chem. B. Volume: 115 Pages: 4184-4194
  • DOI: DOI:10.1021/jp111306k
• [Journal Article] ライン走査型2光子励起蛍光スペクトル顕微鏡を用いた植物細胞中の葉緑体内部微細構造観察 (2011)
  • Author(s): 熊崎茂一, 長谷川慎
  • Journal Title: 分光研究 Volume: 60(1) Pages: 19-21
• [Journal Article] Selective Excitation of Photosystems in Chloroplasts inside Plant Leaves Observed by Near-Infrared Laser-Based Fluorescence Spectral Microscopy (2010)
  • Author(s): Makoto Hasegawa, Takashi Shiina, Masahide Terazima and *Shigeichi Kumazaki
  • Journal Title: Plant Cell Physiol. Volume: 51 Pages: 225-238
  • DOI: doi:10.1093/pcp/pcp182
• [Journal Article] A line-scanning semi-confocal multi-photon fluorescence microscope with a simultaneous broadband spectral acquisition and its application to the study of the thylakoid membrane of a cyanobacterium Anabaena PCC7120 (2007)
  • Author(s): Shigeichi. Kumazaki, Makoto Hasegawa, Mohammad Ghoneim, Yugo Shimizu, Kenji Okamoto, M. Nishiyama, H. Oh-oka and M. Terazima
  • Journal Title: J. Microsc. Volume: 228 Pages: 240-254
  • DOI: DOI:10.1111/j.1365-2818.2007.01835.x
• [Presentation] アンチストークス蛍光の特性 : 異種葉緑体識別能力と人工的クロロフィル会合体検出 (2012)
  • Author(s): 熊崎茂一
  • Organizer: 分子科学討論会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 20120920-23
• [Presentation] 顕微蛍光スペクトル測定で捉えたヘテロシスト形成過程のチラコイド膜変化 (2012)
  • Author(s): 熊崎茂一
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 京都産業大学
  • Year and Date: 2012-03-16
• [Presentation] Simultaneous Sensing of Photosynthetic Activity and Thylakoid Morphology Realized by Fluorescence and Absorption Spectral Microscopy (2010)
  • Author(s): S. Kumazaki
  • Organizer: The 70th Okazaki International Conference on Molecular mechanism of photosynthetic energy conversion: the present research and future prospects
  • Place of Presentation: Okazaki, Aichi, Japan
  • Year and Date: 20101204-1206
• [Presentation] 葉緑体内部構造を見るための2光子励起蛍光スペクトル顕微鏡の諸様式 (2009)
  • Author(s): 熊崎茂一
  • Organizer: 日本分光学会生細胞分光部会平成21年度 生細胞分光部会シンポジウム
  • Place of Presentation: 首都大学
  • Year and Date: 2009-12-04
• [Presentation] 顕微蛍光スペクトルで解析する葉緑体とシアノバクテリアのチラコイド膜自家蛍光 (2008)
  • Author(s): 熊崎茂一
  • Organizer: 奈良先端科学技術大学院大学主催 シンポジウム「見る生物学3」
  • Year and Date: 20081120-21
• [Presentation] Micro-regulation of oxygenic photosynthesis unveiled by fluorescence microspectroscopy (2007)
  • Author(s): S. Kumazaki
  • Organizer: The 67th Okazaki Conference, at Okazaki Conference Center, Molecular Science and Chemical Biology of Biomolecular Function
  • Year and Date: 20071110-12
• [Book] Three-Dimensional High-resolution Microspectroscopic Study of Environment-Sensitive Photosynthetic Membranes”(Ed. by H.Fukumura, M.Irie, Y.Iwasawa, H.Masuhara and K.Uosaki)in Molecular Nano dynamics, Volume 2 Chapter 30 (2009)
  • Author(s): Shigeichi Kumazaki, Makotoh Hasegawa, Mohammad Ghoneim, Takahiko Yoshida, Masahide Terazima, Takashi Shiina, Isamu Ikegam
  • Total Pages: 589-606
  • Publisher: Wiley-VCH
• [Book] 「葉緑体の内部をレーザーで探る」(章著)「反応すれば形が変わるナノの世界～細胞から結晶まで～」 (2009)
  • Author(s): 熊崎茂一
  • Total Pages: 108-117
  • Publisher: クバプロ
• [Book] A Line-Scanning Multiphoton Fluorescence Spectromicroscope Applied to the Study of the Thylakoid Membrane in Chloroplasts”, Photosynthesis. Energy from the Sun, (Ed. by Allen JF, Gantt E, Golbeck JH, and Osmond B.), Volume I, section 7 (2008)
  • Author(s): Shigeichi. Kumazaki, Makotoh. Hasegawa, Takahiko Yoshida, Tarou Taniguchi, Takashi Shiina and Isamu Ikegami
  • Total Pages: 787-790
  • Publisher: Springer, Dordrecht, The Netherlands