Analysis of elimination of non-self and development of Stealth T cells aiming generalized antigen-specific adoptive T cell therapy

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04918
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: Nagasaki University (2016-2017); Mie University (2015)
• Principal Investigator: IKEDA Hiroaki 長崎大学, 医歯薬学総合研究科(医学系), 教授 (40374673)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 細胞療法 / 遺伝子治療 / がん / 非自己細胞 / TCR / MHC

Outline of Final Research Achievements

We successfully generated 'Stealth T cells' that lack MHC class I expression as well as endogenous T cell receptor expression along with enforced expression of NY-ESO-1-specific T cell receptor utilizing beta 2 micro globulin-targetted CRISPR-Cas9 and siTCR vector. We showed that these 'Stealth T cells' lost the ability to stimulate allogeneic T cells while maintaining the anti-tumor activity against NY-ESO-1 positive tumors. These results suggested that 'Stealth T cells' will be useful for the adoptive T cell therapy with allogeneic T cells for cancer patients.

Free Research Field

腫瘍免疫学、細胞療法学、遺伝子治療学