Analyses of physiological role of circadian clock in the ovary

2009 Fiscal Year Final Research Report

• Project/Area Number: 19599001
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental physiology (including Physical medicine and Nutritional physiology)
• Research Institution: Hokkaido University
• Principal Investigator: NAKAMURA Tomoko (吉川 朋子) Hokkaido University, 大学院・医学研究科, 特任助教 (30451397)
• Co-Investigator(Kenkyū-buntansha): NAKAMURA Atsushi 電気通信大学, 電気通信学部, 助教 (50361829)
• Project Period (FY): 2007 – 2009
• Keywords: 概日リズム / 末梢時計 / 中枢時計 / 卵巣 / 黄体形成ホルモン / LHサージ / ルシフェラーゼレポーター / 発情周期

Research Abstract

Current understanding in the field is that the circadian clock in the suprachiasmatic nucleus controls the phase of circadian clock in the peripheral organs. However, the mechanism of the control is remained to be shown. In this study, we used the rat ovary as a model of the peripheral organ, and investigated the potency of LH to control the phase of the circadian clock. When LH level was kept at a constant level in the rat by a pharmacological or surgical method, the amplitude of circadian oscillation was significantly decreased. The result indicates that LH is important to maintain circadian oscillation in the ovary.

Research Products

• [Journal Article] A circadian egg timer gates ovulation (2010)
  • Author(s): Sellix, M. T., Yoshikawa, T., Menaker, M.
  • Journal Title: Curr. Biol. 20 Pages: R266-267
  • Peer Reviewed
• [Journal Article] Ontogeny of circadian organization in the rat (2009)
  • Author(s): Yoshikawa T., Yamazaki S., Biscoe E. W., Numano R., Gallaspy L. M., Soulsby S., Papadimas E., Pezuk P., Doyle S. E., Tei H., Sakaki Y., Block G. D., Menaker M.
  • Journal Title: J. Biol. Rhythms 24 Pages: 55-63
  • Peer Reviewed
• [Journal Article] The timing of the ovarian circadian clock is regulated by gonadotrophins (2009)
  • Author(s): Yoshikawa T., Sellix M., Pezuk P., Menaker M.
  • Journal Title: Endocrinology 50 Pages: 4338-4347
  • Peer Reviewed
• [Journal Article] Retinal pathways influence temporal niche (2008)
  • Author(s): Doyle S. E., Yoshikawa T., Hillson H., Menaker M.
  • Journal Title: Proc. Natl. Acad. Sci. 105 Pages: 13133-13138
  • Peer Reviewed
• [Journal Article] Clock mechanisms for seasonal adaptation : Morning and evening oscillators in the suprachiasmatic nucleus (2008)
  • Author(s): Honma S., Inagaki N., Ono D., Yoshikawa T., Hashimoto S., Honma K.
  • Journal Title: Sleep and Biological Rhythms 6 Pages: 84-90
  • Peer Reviewed
• [Journal Article] 生体リズムの分子機構 (2008)
  • Author(s): 吉川朋子・本間さと・本間研一
  • Journal Title: 日本臨床 66(S2) Pages: 90-95
• [Presentation] Photoperiodic response of multiple oscillators in mouse SCN (2009)
  • Author(s): 吉川朋子・本間さと・本間研一
  • Organizer: 第16回日本時間生物学会学術大会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 20091000
• [Presentation] 卵巣に存在する概日時計の位相調節機構の解明 (2009)
  • Author(s): 吉川朋子
  • Organizer: 第80回日本動物学会大会
  • Place of Presentation: グランシップ静岡
  • Year and Date: 20090900
• [Presentation] 母子分離中の環境温度が新生ラットの概日リズムに及ぼす影響 (2009)
  • Author(s): 吉川朋子・松野亜美・山仲勇二郎・西出真也・本間さと・本間研一
  • Organizer: 日本動物学会第80回日本動物学会大会
  • Place of Presentation: グランシップ静岡
  • Year and Date: 20090900
• [Presentation] Effects of environmental temperature during maternal deprivation on circadian rhythms of neonatal rats (2009)
  • Author(s): 吉川朋子・松野亜美・山仲勇二郎・西出真也・本間さと・本間研一
  • Organizer: International Sapporo Symposium on Biological Rhythm
  • Place of Presentation: 札幌 北海道大学
  • Year and Date: 20090800
• [Presentation] Effects of ambient temperature during maternal deprivation on circadian rhythms of neonatal rats (2009)
  • Author(s): 吉川朋子・松野亜美・山仲勇二郎・西出真也・本間さと・本間研一
  • Organizer: XXXVI International Congress of Physiological Science
  • Place of Presentation: 京都国際会議場
  • Year and Date: 20090700
• [Presentation] Phase control of the ovarian circadian clock by the central clock (2008)
  • Author(s): 吉川朋子・本間さと・本間研一・Michael Menaker
  • Organizer: 第85回日本生理学会大会
  • Place of Presentation: 東京 京王プラザホテル
  • Year and Date: 20080300
• [Presentation] Ontogeny of circadian organization inrat (2007)
  • Author(s): 吉川朋子・山崎晋・Elizabeth W. Biscoe・沼野利佳・Lauren M. Gallaspy・Stacy Soulsby・Evagelia Papadimas・Pinar Pezuk・Susan Doyle・程肇・榊佳之・Gene D. Block・Michael Menaker
  • Organizer: 2^<nd> World Congress of Chronobiology
  • Place of Presentation: 東京 京王プラザホテル
  • Year and Date: 20071100
• [Presentation] Perl-luc oscillations and localizations of neuropeptides in the suprachiasmatic nucleus (2007)
  • Author(s): 吉川朋子・本間さと・本間研一
  • Organizer: 第14回日本時間生物学会学術大会
  • Place of Presentation: 東京 京王プラザホテル
  • Year and Date: 20071100
• [Book] Molecular Imaging for Integrated Medical Therapy and Drug Development (Tamai N., Kuge Y. Eds.) Bioluminescent imaging for assessing heterogeneous cell function in the mammalian central circadian clock (2009)
  • Author(s): Honma S., Yoshikawa T., Nishide S.-H., Ono D., Honma K.-I.
  • Total Pages: 189-196
  • Publisher: Springer
• [Book] 視交叉上核と光周性 海老原史樹文 (編) 光周性の分子生物学 (2009)
  • Author(s): 吉川朋子・本間さと
  • Total Pages: 159-168
  • Publisher: シュプリンガー・ジャパン
• [Book] Roles of melatonin in photoperiodic gonadal response of birds Experimental Endocrinology and Reproductive Biology (Haldar C., Singaravel M., Pandi-Perumal S. R., Cardinali D. P. Eds.) (2008)
  • Author(s): Yoshikawa T., Iigo M., Okano T., Fukada Y.
  • Total Pages: 85-102
  • Publisher: Science Publishers