2021 Fiscal Year Final Research Report
Function and formation of exosome-like membrane vesicles of nitrogen-fixing bacterium
| Project/Area Number |
19K23665
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund |
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| Review Section |
0601:Agricultural chemistry and related fields
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2019-08-30 – 2022-03-31
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| Keywords | 膜小胞 / 窒素固定細菌 / プロテオーム解析 / X線結晶構造解析 |
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| Outline of Final Research Achievements |
This study deals with outer membrane vesicles (OMVs) which are formed and secreted from the extracellular membrane of Gram-negative bacteria. Based on the characteristics of gene knockout strains of Azotobacter vinelandii, function-unknown S-layer protein and a flagellar fiber protein FliC homolog protein were found to be involved in membrane formation and OMV production, respectively. Proteomic analysis of the OMV identified 640 proteins, providing clues to predict the mechanism of protein transport to OMV and the bioactivities of OMV.
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| Free Research Field |
農芸化学および応用微生物学
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| Academic Significance and Societal Importance of the Research Achievements |
細菌が自身の細胞膜から形成・分泌する膜小胞は細胞間コミュニケーションなど様々な生理活性を示すことが明らかになってきた一方、その普遍的な形成機構は不明であった。本研究では窒素固定グラム陰性細菌Azotobacter vinelandiiに着目し、膜小胞に内包されるタンパク質の網羅的解析を行うとともに、膜小胞の形成に関わる候補タンパク質を見出した。本研究成果は、膜小胞の普遍的な形成機構の一端を明らかにすることのみならず、OMVのサイズや性質を制御する分子機構の理解、及び窒素固定細菌OMVの新たな機能の発見に繋がることが期待される。
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