2010 Fiscal Year Final Research Report
Lambda-DSLM, a new light-sheet microscope for spectroscopy
| Project/Area Number |
21770177
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | National Institute for Basic Biology |
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Principal Investigator |
NONAKA Shigenori National Institute for Basic Biology, 時空間制御研究室, 准教授 (90435529)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 発生・分化 / 細胞・組織 / 共焦点顕微鏡 |
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| Research Abstract |
Light-sheet microscopy including Digital Scanned Light-sheet Microscope (DSLM) has advantages with its deep penetration and low photo-toxicity. We have developed a new microscope system combining spectroscopy with DSLM, namely lambda-DSLM. So far we have confirmed that the system has enough spectral resolution for linear unmixing or fluorescence or raman imaging, via analyses using fluorescent beads or emission lines from light sources. Imaging of biological specimen expressing fluorescent proteins and development of an integrated software for device control and image reconstruction are ongoing.
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