2010 Fiscal Year Final Research Report

Elucidating signaling components under low potassium conditions

Research Project

Project/Area Number	21870045
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Plant molecular biology/Plant physiology
Research Institution	The Institute of Physical and Chemical Research
Principal Investigator	SHIN Ryoung The Institute of Physical and Chemical Research, 機能調節研究ユニット, ユニットリーダー (80525577)
Project Period (FY)	2009 – 2010
Keywords	カリウムトランスポーター / FOXライブラリ / 栄養欠乏 / 非生物的ストレス / 転写因子
Research Abstract	Arabidopsis FOX (Full-length cDNA over-expressor genes) agrobacterium librarys were transformed into established AtHAK5 promoter::luciferase Arabidopsis plants. These AtHAK5 promoter::luciferase plants with FOX library have been screened and more than 200 candidate transgenic plants that have higher luciferase activity under full nutrient condition have been isolated. Among them, fifteen lines were analyzed to determine which gene is overexpressed, and some candidates were analyzed for their expression in response to nutrient deficiency. The overexpression plants and knockout plants of WRKY TF were obtained. The overexpression plants showed increased tolerance to potassium deficient condition.