Mechanisms of blastema formation after leg amputationin Gryllus bimaculatus

2012 Fiscal Year Final Research Report

• Project/Area Number: 22370080
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: The University of Tokushima
• Principal Investigator: NOJI Sumihare 徳島大学, 本部, 理事 (40156211)
• Co-Investigator(Kenkyū-buntansha): OHUCHI Hideyo 岡山大学, 医歯薬学総合研究科, 教授 (00253229) ; MITO Taro 徳島大学, 大学院・ソシオテクノサイエンス研究部, 助教 (80322254)
• Project Period (FY): 2010 – 2012
• Keywords: コオロギ / 脚再生 / ゲノム編集技術 / Jak/Stat 系 / ノックアウトコオロギ

Research Abstract

We attempted to elucidate molecular mechanisms underlying leg regeneration of the cricket nymph. We obtained cricket genome data, using new sequencers to know basic genetic data. We also developed a new method to edit cricket genome by artificial nucleases such as TALEN or CRISPR/Cas. We made successfully knockout crickets. We found that the Jak/Stat signaling system is involved in formation of blastema. With our results, we may facilitate elucidation of regeneration mechanisms in general

Research Products

• [Journal Article] Analysis of RNA-Seq data reveals involvement of JAK/STAT signalling during leg regeneration in the cricket Gryllus bimaculatus.Development (2013)
  • Author(s): Bando T, Ishimaru Y, Kida T, Hamada Y, Matsuoka Y, Nakamura T, Ohuchi H, Noji S, Mito T
  • Journal Title: Select item Volume: 140 Pages: 959-964
  • DOI: DOI:10.1242/dev.084590.
  • Peer Reviewed
• [Journal Article] Non-transgenic genome modifications in a hemimetabolous insect using zinc-finger and TAL effector nucleases (2012)
  • Author(s): Watanabe T, Ochiai H, Sakuma T, Horch HW, Hamaguchi N, Nakamura T, Bando T,Ohuchi H, Yamamoto T, Noji S, Mito T.
  • Journal Title: Nat Commun Volume: 3 Pages: e1017.
  • DOI: DOI:10.1038/ncomms2020.
  • Peer Reviewed
• [Journal Article] Functional analysis of the role of eyes absent and sine oculis in the developing eye of the cricket Gryllus bimaculatus (2012)
  • Author(s): Takagi A, Kurita K, Terasawa T, Nakamura T, Bando T, Moriyama Y, Mito T, Noji S, Ohuchi H
  • Journal Title: Dev Growth Differ Volume: 54 Pages: 227-240
  • DOI: DOI:10.1111/j.1440-169X
  • Peer Reviewed
• [Journal Article] Ancestral functions of Delta/Notch signaling in the formation of body and leg segments in the cricket Gryllus bimaculatus. (2011)
  • Author(s): Mito T, Shinmyo Y, Kurita K, Nakamura T, Ohuchi H, Noji S
  • Journal Title: Development. Volume: 138 Pages: 3823-3833
  • DOI: DOI:10.1242/dev.060681
  • Peer Reviewed
• [Journal Article] Cricket body size is altered by systemic RNAi against insulin signaling components and epidermal growth factor receptor (2011)
  • Author(s): Dabour N, Bando T, Nakamura T, Miyawaki K, Mito T, Ohuchi H, Noji S
  • Journal Title: Dev Growth Di Volume: 53 Pages: 857-869
  • DOI: DOI:10.1111/j.1440-169X
  • Peer Reviewed
• [Journal Article] a mammalian Lix1 homologue, regulates leg size and growth under the Dachsous/Fat signaling pathway during tissue regeneratio (2011)
  • Author(s): Bando T, Hamada Y, Kurita K, Nakamura T, Mito T, Ohuchi H, Noji S. Lowfat
  • Journal Title: Dev Dyn Volume: 240 Pages: 1440-1453
  • DOI: DOI:10.1002/dvdy.22647.
  • Peer Reviewed
• [Journal Article] Regulation of leg size and shape: involvement of the Dachsousfat signaling pathwa (2011)
  • Author(s): Bando T, Mito T, Nakamura T, Ohuchi H, Noji S
  • Journal Title: Dev Dyn Volume: 240 Pages: 1028-1041
  • DOI: DOI:10.1002/dvdy.22590
  • Peer Reviewed
• [Journal Article] Imaging of transgenic cricket embryos reveals cell movements consistent with a syncytial patterning mechanism (2010)
  • Author(s): Nakamura T, Yoshizaki M, Ogawa S, Okamoto H, Shinmyo Y, Bando T, Ohuchi H, Noji S, Mito T
  • Journal Title: Curr Biol Volume: 18 Pages: 1641-1647
  • DOI: DOI:10.1016/j.cub.2010.07.044
  • Peer Reviewed