Detection of structural change of DNA using disulfide bond formation and its application to elucidation of molecular recognition mechanisms

2014 Fiscal Year Final Research Report

• Project/Area Number: 24310158
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Living organism molecular science
• Research Institution: Osaka University
• Principal Investigator: IWAI Shigenori 大阪大学, 基礎工学研究科, 教授 (10168544)
• Co-Investigator(Renkei-kenkyūsha): SUGASAWA Kaoru 神戸大学, バイオシグナル研究センター, 教授 (70202124) ; KOJIMA Chojiro 大阪大学, 蛋白質研究所, 准教授 (50333563)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: ＤＮＡ / 構造変化 / ジスルフィド結合 / ＨＰＬＣ / タンパク質 / 分子認識

Outline of Final Research Achievements

To detect the DNA helix bending by a chemical approach, duplexes containing 2-O-mercaptoalkyl-β-D-arabinofuranose on both sides of the major groove were synthesized, and the effects of the cisplatin adduct, the abasic site analog, and the (6-4) photoproduct on DNA structures were investigated. A disulfide bond was formed depending on the cisplatin adduct, and dynamic DNA bending was shown for the abasic site and the (6-4) photoproduct. Using the bent structure obtained by this method, the model proposed for the handover of damaged DNA from the UV-DDB protein to the XPC protein in the nucleotide excision repair was validated.

Free Research Field

生物有機化学