| Project/Area Number |
15K19100
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
Tsukamoto Yumiko 国立感染症研究所, ハンセン病研究センター 感染制御部, 主任研究官 (50554507)
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| Project Period (FY) |
2015-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | BCG / 結核菌 / 潜伏感染 / ワクチン |
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| Outline of Final Research Achievements |
In this study, I inoculated mice with recombinant BCG, BCG-PHM. BCG-PHM is known to have high vaccine efficacy against tuberculosis. After mice were infected with tuberculosis, I purifired RNA from lungs of these mice. I then analysed gene expression by tuberculosis and by infected host cells.
From tuberculosis-infected murine lungs, I obtained only little amount of RNA derived from tuberculosis. Therefore, it was difficult to analysis the expression of many kinds of genes. However, it was suggested that the dormancy-related gene was upregulated in tuberculosis which infected BCG-PHM-vaccinated mice. It was also revealed that strong immune response was induced by tuberculosis infection after the inoculation of BCG-PHM in murine lungs.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は、BCG接種を行ったマウス肺中に存在する結核菌が休眠状態に移行しているか解析する目的で実施された。結核菌に感染したヒトでは、免疫系により結核菌の増殖が抑制されるものの結核菌の一部は休眠状態となる。休眠状態の結核菌は肉芽腫と呼ばれる構造の中に存在し、免疫細胞からの攻撃を受けにくい。
本研究においてBCG接種後に結核菌を感染させたマウスの肺中に存在する結核菌は休眠状態に移行していることが示唆されたため、抗結核菌ワクチンの開発には休眠状態に移行する結核菌への対応を考慮する必要があることが推測できた。
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