Creation of hepatic tissue containning Human Induced Pluripotent Stem Cells derived hepatocyte like cells

• Project/Area Number: 15K19863
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: Keio University
• Principal Investigator: Takagi Chisato 慶應義塾大学, 医学部(信濃町), 助教 (10626708)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
• Keywords: iPS細胞 / 間葉系幹細胞 / 脱細胞化肝骨格 / 肝細胞 / iPS細胞由来肝細胞 / 脱細胞化肝臓 / iPS由来肝細胞

Outline of Final Research Achievements

To obtain more mature iPS induced hepatocyte like cells, we investigated a novel protocol by using mesenchymal stem cells and decellularized liver matrix. Mesenchymal stem cells and decellularized liver matrix could enhance the hepatic function of human iPSC-derived hepatocyte-like cells. However, these cells have immature function compared to primary hepatocyte. More technological innovations are needed to clinical application.

Research Products

• [Journal Article] Mesenchymal Stem Cells Contribute to Hepatic Maturation of Human Induced Pluripotent Stem Cells Chisato (2017)
  • Author(s): Chisato Takagi, Hiroshi Yagi, Makiko Hieda, Kazuki Tajima, Taizo Hibi, Yuta Abe, Minoru Kitago, Masahiro Shinoda, Osamu Itano, Yuko Kitagawa
  • Journal Title: European Surgical Research Volume: 58 Issue: 1-2 Pages: 27-39
  • DOI: 10.1159/000448516
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 間葉系幹細胞はより成熟したiPS細胞由来肝細胞への分化誘導を可能にする (2015)
  • Author(s): 高木 知聡、八木 洋、板野 理、篠田 昌宏、北郷 実、阿部 雄太、日比 泰造、北川 雄光
  • Organizer: 第23回 日本消化器関連学会週間
  • Place of Presentation: グランドプリンスホテル高輪（東京都港区）
  • Year and Date: 2015-10-08
• [Presentation] MESENCHYMAL STEM CELLS CONTRIBUTE TO HEPATIC MATURATION OF INDUCED PLURIPOTENT STEM CELLS (2015)
  • Author(s): Chisato Takagi, Hiroshi Yagi, Kazuki Tajima, Kenta Inomata, Taizo Hibi, Yuta Abe, Minoru Kitago, Masahiro Shinoda, Osamu Itano, Yuko Kitagawa
  • Organizer: ISSCR(International society of stem cell reserch) 2015 annual meeting
  • Place of Presentation: ストックホルム（スウェーデン）
  • Year and Date: 2015-06-25
  • Int'l Joint Research
• [Presentation] iPS細胞由来肝細胞を用いた肝臓器再生の試み (2015)
  • Author(s): 高木 知聡、八木 洋、板野 理、篠田 昌宏、北郷 実、阿部 雄太、日比 泰造、北川 雄光
  • Organizer: 第27回 日本肝胆膵外科学会
  • Place of Presentation: ホテルグランパシフィックLE DAIBA（東京都港区）
  • Year and Date: 2015-06-12