Pathological analysis of Graves' disease using highly sensitive thyroid stimulating antibody assay

• Project/Area Number: 17K08982
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Osaka University
• Principal Investigator: HIDAKA YOH 大阪大学, 医学系研究科, 准教授 (30243231)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: バセドウ病 / 甲状腺刺激抗体

Outline of Final Research Achievements

Autoantibodies (TSAbs) that stimulate the thyroid present in the blood of Graves 'disease patients are responsible for the Graves' disease. Since it is difficult for humans to immediately try a new treatment for Graves' disease, it is necessary to examine it in vitro. Therefore, lymphocytes in the blood of patients with Graves' disease were cultured, and it was examined whether or not TSAb could be detected in the culture medium by a highly sensitive measurement method. An increase in cyclic AMP, which is an index of TSAb, could be confirmed in the culture medium.

Academic Significance and Societal Importance of the Research Achievements

これまでは、バセドウ病患者の血液中のリンパ球を培養しても培養液中に甲状腺を刺激する自己抗体（ＴＳＡｂ）を検出できなかったため、バセドウ病の新たな治療薬の候補を体外で検討することはできなかった。今回の研究で、十分ではないもののＴＳＡｂの指標となるサイクリックＡＭＰの増加を培養液中で確認できたため、将来、体外の検討でＴＳＡｂの産生を抑制できた治療薬のみを人で投与することができるようになると期待される。

Research Products

• [Journal Article] Association of CD58 Polymorphisms and its Protein Expression with the Development and Prognosis of Autoimmune Thyroid Diseases. (2020)
  • Author(s): Yamamoto M, Watanabe M, Inoue N, Watanabe A, Ozaki H, Ohsaki M, Hidaka Y, Iwatani Y.
  • Journal Title: Immunol Invest Volume: 49(1-2) Issue: 1-2 Pages: 106-119
  • DOI: 10.1080/08820139.2019.1659811
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Increases of CD80 and CD86 Expression on Peripheral Blood Cells and their Gene Polymorphisms in Autoimmune Thyroid Disease. (2020)
  • Author(s): Watanabe A, Inoue N, Watanabe M, Yamamoto M, Ozaki H, Hidaka Y, Iwatani Y.
  • Journal Title: Immunol Invest Volume: 49(1-2) Issue: 1-2 Pages: 191-203
  • DOI: 10.1080/08820139.2019.1688343
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] In tube immunocytochemistry for fluorescence-activated cell sorting that prevents RNA degradation in sorted cells (2020)
  • Author(s): Fukano H, Takano T, Fujimoto Y, Nakatani R, Watanabe M, Hidaka Y, Shimomura I
  • Journal Title: Biotech Histochem Volume: 95 Issue: 1 Pages: 1-7
  • DOI: 10.1080/10520295.2019.1632485
  • Peer Reviewed
• [Journal Article] Association of IL6 gene methylation in peripheral blood cells with the development and prognosis of autoimmune thyroid diseases. (2019)
  • Author(s): Hirai N, Watanabe M, Inoue N, Kinoshita R, Ohtani H, Hidaka Y, Iwatani Y.
  • Journal Title: Autoimmunity Volume: 52(7-8) Issue: 7-8 Pages: 251-255
  • DOI: 10.1080/08916934.2019.1669568
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Association of the polymorphisms in Th2 chemotaxis-related genes with the development and prognosis of autoimmune thyroid diseases (2018)
  • Author(s): Omori N, Watanabe M, Inoue N, Taniguchi J, Hidaka Y, Iwatani Y
  • Journal Title: Endocrine Journal Volume: 65 Issue: 8 Pages: 815-826
  • DOI: 10.1507/endocrj.EJ18-0050
  • NAID: 130007458402
  • ISSN: 0918-8959, 1348-4540
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Polymorphisms in Th17-related genes and the pathogenesis of autoimmune thyroid disease (2018)
  • Author(s): Kunisato T, Watanabe M, Inoue N, Okada A, Nanba T, Kobayashi W, Inoue Y, Katsumata Y, Omori N, Nobuhara T, Takemura K, Hidaka Y, Iwatani Y
  • Journal Title: Autoimmunity Volume: 51 Issue: 7 Pages: 360-369
  • DOI: 10.1080/08916934.2018.1534963
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Methylation levels of the TNFA gene are different between Graves’ and Hashimoto’s diseases and influenced by the TNFA polymorphism (2018)
  • Author(s): Morita Emi、Watanabe Mikio、Inoue Naoya、Hashimoto Hidemi、Haga Emi、Hidaka Yoh、Iwatani Yoshinori
  • Journal Title: Autoimmunity Volume: 印刷中 Issue: 3 Pages: 118-125
  • DOI: 10.1080/08916934.2018.1448078
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Polymorphisms and expression of genes encoding Argonautes 1 and 2 in autoimmune thyroid diseases (2018)
  • Author(s): Tokiyoshi Ena、Watanabe Mikio、Inoue Naoya、Hidaka Yoh、Iwatani Yoshinori
  • Journal Title: Autoimmunity Volume: 51 Issue: 1 Pages: 35-42
  • DOI: 10.1080/08916934.2017.1416468
  • Peer Reviewed
• [Journal Article] Functional polymorphisms of the type 1 and type 2 iodothyronine deiodinase genes in autoimmune thyroid diseases (2018)
  • Author(s): Inoue, N., Watanabe, M., Katsumata, Y., Ishido, N., Hidaka, Y., Iwatani, Y.
  • Journal Title: Immunological Investigation Volume: 印刷中
  • Peer Reviewed