Mechanism of the removal of degenerated own cells by Draper, a phagocytosis receptor of Drosophila

• Project/Area Number: 19370051
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Kanazawa University
• Principal Investigator: NAKANISHI Yoshinobu Kanazawa University, 薬学系, 教授 (40172358)
• Co-Investigator(Kenkyū-buntansha): 白土 明子 金沢大学, 医学系研究科, 講師 (90303297)
• Co-Investigator(Renkei-kenkyūsha): SHIRATSUCHI Akiko 金沢大学, 薬学系, 准教授 (90303297)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000); Fiscal Year 2009: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2008: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2007: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
• Keywords: 貪食 / アポトーシス / 自然免疫 / ショウジョウバエ / 細菌感染 / 細胞内情報伝達 / リン酸化 / 小胞体 / 細胞間認識 / ショウジョウバェ

Research Abstract

線虫の貪食受容体CED-1に相当するショウジョウバエのDraperは、血球細胞及びグリア細胞によるアポトーシス細胞の貪食除去に必要とされる。本研究では、Draperが認識するアポトーシス細胞側の目印分子が探索され、研究代表者らがPretaporterと名付けた小胞体タンパク質が見いだされた。Pretaporterはアポトーシス時に小胞体から細胞表層に移動してDraperに認識されるようになると考えられた。また、Pretaporterの結合によりDraperのチロシンリン酸化が促され、それを契機にして細胞内に貪食誘導性の情報が伝達されると示唆された。

Research Products

• [Journal Article] a Drosophila protein serving as a ligand for Draper in the phagocytosis of apoptotic cells (2009)
  • Author(s): Kuraishi, T, Nakagawa, Y., Nagaosa, K., Hashimoto, Y., Ishimoto, T., Moki, T., Fujita, Y., Nakayama, H., Dohmae, N., Shiratsuchi, A., Yamamoto, N., Ueda, K., Yamaguchi, M., Awasaki, T., Nakanishi, Y. Pretaporter
  • Journal Title: EMBO J. 28 Pages: 3868-3878
  • Peer Reviewed
• [Journal Article] A. Identification of lipoteichoic acid as a ligand for Draper in the phagocytosis of Staphylococcus aureus by Drosophila hemocytes (2009)
  • Author(s): Hashimoto, Y., Tabuchi, Y., Sakurai, K., Kutsuna, M., Kurokawa, K., Awasaki, T., Sekimizu, K., Nakanishi, Y., Shiratsuchi
  • Journal Title: J. Immunol. 183 Pages: 7451-7460
  • Peer Reviewed
• [Journal Article] Identification of lipoteichoic acid as a ligand for Draper in the phagocytosis of Staphylococcus aureus by Drosophila hemocytes (2009)
  • Author(s): 橋本優実, 他8名
  • Journal Title: Journal of Immunology 183 Pages: 7451-7560
  • Peer Reviewed
• [Journal Article] Pretaporter, a Drosophila protein serving as a ligand for Draper in the phagocytosis of apoptotic cells (2009)
  • Author(s): 倉石貴透, 他14名
  • Journal Title: EMBO Journal 28 Pages: 3868-3878
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): 中西義信、Peter Henson、白土明子
  • Journal Title: Target Pattern Recognition in Innate Immunity第9章「Pattern recognition in phagocytic clearance of altered self」(Landes Bioscience(米国)) Pages: 129-138
• [Journal Article] Y. Identification of calreticulin as a marker for phagocytosis of apoptotic cells in Drosophila (2007)
  • Author(s): Kuraishi, T., Manaka, J., Kono, M., Ishii, H., Yamamoto, N., Koizumi, K., Shiratsuchi, A., Lee, B.L., Higashida, H., Nakanishi
  • Journal Title: Exp. Cell Res. 313 Pages: 500-510
  • Peer Reviewed
• [Presentation] リポタイコ酸とDraperを介したショウジョウバエヘモサイトによる黄色ブドウ球菌の貪食 (2009)
  • Author(s): 橋本優実、田淵幸幾、黒川健児、粟崎健、関水和久、中西義信、白土明子
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2009-10-23
• [Presentation] 黄色ブドウ球菌のD-アラニン化壁タイコ酸によるショウジョウバエでの抗菌ペプチド産生の抑制 (2009)
  • Author(s): 田淵幸幾
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2009-10-23
• [Presentation] Involvement of a core protein of Drosophila Perlecan in the phagocytosis of apoptotic cells (2009)
  • Author(s): 岡田亮
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2009-10-23
• [Presentation] アポトーシス細胞貪食におけるショウジョウバエβν integrinの役割 (2009)
  • Author(s): 永長一茂
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2009-10-23
• [Presentation] Role of Pretaporter as a ligand for Draper in phagocytosis of apoptotic cells by Drosophila hemocytes (2009)
  • Author(s): Y. Fujita, Y. Nakagawa, T. Kuraishi, Y. Hashimoto, R. Okada, A. Shiratsuchi, Y. Nakanishi
  • Organizer: 17th International Symposium on Molecular Cell Biology of Macrophage
  • Place of Presentation: KKRホテル金沢(石川県)
  • Year and Date: 2009-07-03
• [Presentation] Draper-mediated phagocytosis of Staphylococcus aureus by Drosophila hemocytes (2009)
  • Author(s): 橋本優実
  • Organizer: 17^<th> International Symposium on Molecular Cell Biology of Macrophages
  • Place of Presentation: KKRホテル金沢(石川県)
  • Year and Date: 2009-07-03
• [Presentation] Role of Pretaporter as a ligand for Draper in phagocytosis of apoptotic cells by Drosophila hemocytes (2009)
  • Author(s): 藤田融
  • Organizer: 17^<th> International Symposium on Molecular Cell Biology of Macrophages
  • Place of Presentation: KKRホテル金沢(石川県)
  • Year and Date: 2009-07-03
• [Presentation] Involvement of a core protein of Drosophila Perlecan in the phagocytosis of apoptotic cells (2009)
  • Author(s): 岡田亮
  • Organizer: 17^<th> International Symposium on Molecular Cell Biology of Macrophages
  • Place of Presentation: KKRホテル金沢(石川県)
  • Year and Date: 2009-07-03
• [Presentation] Mechanism and role of Draper-mediated phagocytosis of Staphylococcus aureus by Drosophila hemocytes (2009)
  • Author(s): A. Shiratsuchi
  • Organizer: Gordon Research Conferences: Apoptotic Cell Recognition & Clearance
  • Place of Presentation: Colby-Sawyer College(米国)
  • Year and Date: 2009-07-02
• [Presentation] Mechanism and role of Draper-mediated phagocytosis of Staphylococcus aureus by Drosophila hemocytes (2009)
  • Author(s): 白土明子
  • Organizer: Gordon Research Conferences
  • Place of Presentation: Colby-Sawyer College(USA)
  • Year and Date: 2009-07-02
• [Presentation] Gordon Research Conferences (2009)
  • Author(s): T. Kuraishi、Pretaporter, a Draper ligand
  • Organizer: Apoptotic Cell Recognition & Clearance
  • Place of Presentation: Colby-Sawyer College(米国)
  • Year and Date: 2009-06-30
• [Presentation] Pretaporter, a Draper ligand (2009)
  • Author(s): 倉石貴透
  • Organizer: Gordon Research Conferences
  • Place of Presentation: Colby-Sawyer College(USA)
  • Year and Date: 2009-06-30
• [Presentation] Pretaporter, a Drosophila endoplasmic reticulum protein serving as a ligand for Draper in phagocytosis of apoptotic cells (2009)
  • Author(s): T. Kuraishi, Y. Nakagawa, T. Ishimoto, T. Moki, Y. Fujita, H. Nakayama, N. Dohmae, A. Shiratsuchi, Y. Hashimoto, N. Yamamoto, K. Ueda, M. Yamaguchi, T. Awasaki, Y. Nakanishi
  • Organizer: Jacques Monod Conferences
  • Place of Presentation: Aussoisホール(フランス)
  • Year and Date: 2009-05-24
• [Presentation] ショウジョウバエヘモサイトによるDraperを介した細菌の貪食機構 (2009)
  • Author(s): 橋本優実
  • Organizer: 日本生化学会北陸支部第27回大会
  • Place of Presentation: 福井大学(福井県)
  • Year and Date: 2009-05-23
• [Presentation] ショウジョウバエCed-6のアポトーシス細胞貪食における役割 (2008)
  • Author(s): 倉石貴透、真中純子、粟崎健、中川祐紀子、荒井國三、岡田亮、白土明子、中西義信
  • Organizer: 第81回日本生化学会大会
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2008-12-10
• [Presentation] ショウジョウバエCed-6のアポトーシス細胞貪食における役割 (2008)
  • Author(s): 倉石貴透
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸市
  • Year and Date: 2008-12-10
• [Presentation] ショウジョウバエ貪食受容体が認識するアポトーシス細胞側リガンドの同定 (2008)
  • Author(s): 中西義信、倉石貴透、石本聖、茂木武、中川祐紀子、中山洋、堂前直、白土明子、橋本優実、山本奈央子、上田晃一、山口政光、粟崎健
  • Organizer: 第17回日本アポトーシス研究会学術集会
  • Place of Presentation: メルパルク京都(京都府)
  • Year and Date: 2008-08-01
• [Presentation] ショウジョウバエ貪食受容体が認識するアポトーシス細胞側リガンドの同定 (2008)
  • Author(s): 中西義信
  • Organizer: 第17回日本アポトーシス研究会学術集会
  • Place of Presentation: 京都市
  • Year and Date: 2008-08-01
• [Presentation] Pretaporter, a Drosophila endoplasmic reticulum protein serving as a ligand for draper in phagocytosis of apoptotic cells (2008)
  • Author(s): T. Kuraishi, T. Ishimoto, T. Moki, Y. Nakagawa, H. Nakayama, A. Shiratsuchi, Y. Hashimoto, N. Yamamoto, K. Ueda, M. Yamaguchi, T. Awasaki, Y. Nakanishi
  • Organizer: The Seventh International Cell Death Society Symposium
  • Place of Presentation: Shanghai National Accounting Institute(中国)
  • Year and Date: 2008-06-06
• [Presentation] Pretaporter, a Drosophila endoplasmic reticulum protein serving as a ligand for draper in phagocytosis of apoptotic cells (2008)
  • Author(s): Takayuki Kuraishi
  • Organizer: The Seventh International Cell Death Society Symposium on Targeting cell death pathways for human diseases
  • Place of Presentation: Shanghai Mega City, China
  • Year and Date: 2008-06-06
• [Presentation] Phagocytic removal of apoptotic cells in Drosophila: identification of a receptor and ligand (2008)
  • Author(s): Y. Nakanishi
  • Organizer: Spring International Convention of the Pharmaceutical Society of Korea
  • Place of Presentation: International Convention Center Jeju(韓国)
  • Year and Date: 2008-05-01
• [Presentation] Phagocytic removal of apoptotic cells in Drosophila : identification of a receptor and ligand (2008)
  • Author(s): Yoshinobu Nakanishi
  • Organizer: 2008 spring International Convention of the Pharmaceutical Society of Korea
  • Place of Presentation: Jeju, Republic of Korea
  • Year and Date: 2008-05-01
• [Presentation] Characterization of CG1837 protein as a candidate ligand for Draper, a Drosophila phagocytosis receptor (2008)
  • Author(s): T. Kuraishi, T. Ishimoto, N. Yamamoto, K. Ueda, H. Nakayama, M. Yamaguchi, T. Awasaki, Y. Hashimoto, T. Moki, A. Shiratsuchi, Y. Nakanishi
  • Organizer: 49th Annual Drosophila Research Conference
  • Place of Presentation: Town and Country Resort & Convention Center(米国)
  • Year and Date: 2008-04-04
• [Presentation] Characterization of CG1837 protein as a candidate ligand for Draper, a Drosophila phagocytosis receptor (2008)
  • Author(s): Takayuki Kuraishi
  • Organizer: 49^<th> Annual Drosophila Research Conference
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2008-04-04
• [Presentation] ショウジョウバエ貪食受容体Draperが認識するアポトーシス細胞表層分子の同定 (2007)
  • Author(s): 倉石貴透, 山本奈央子, 上田晃一, 中山洋, 橋本優実, 白土明子, 中西義信
  • Organizer: 第80回日本生化学会大会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2007-12-12
• [Presentation] Drosophilaのアポトーシス細胞貪食におけるインテグリンの役割 (2007)
  • Author(s): 荒井 國三
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会
  • Place of Presentation: 横浜市
  • Year and Date: 2007-12-12
• [Presentation] ショウジョウバエ貪食受容体Draperが認識するアポトーシス細胞表層分子の同定 (2007)
  • Author(s): 倉石 貴透
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会
  • Place of Presentation: 横浜市
  • Year and Date: 2007-12-12
• [Presentation] ショウジョウバエ感染症モデルを用いた細菌病原性と宿主免疫応答の解析 (2007)
  • Author(s): 中西義信
  • Organizer: 日本薬学会関東支部第32回学術講演会
  • Place of Presentation: 日本薬学会長井記念会館長井記念ホール(東京都)
  • Year and Date: 2007-10-20
• [Presentation] ショウジョウバエ感染症モデルを用いた細菌病原性と宿主免疫応答の解析 (2007)
  • Author(s): 中西 義信
  • Organizer: 日本薬学会関東支部第32回学術講演会
  • Place of Presentation: 東京都
  • Year and Date: 2007-10-20
• [Presentation] Hashimoto, H. Nakayama, A. Shiratsuchi, Y. Nakanishi、Isolation of an endoplasimic reticulum protein as a candidate ligand for Draper, a Drosophila phagocytosis receptor (2007)
  • Author(s): T. Kuraishi, N. Yamamoto, K. Ueda, Y. Hashimoto, H. Nakayama, A. Shiratsuchi, Y. Nakanishi
  • Organizer: 第8回日本ショウジョウバエ研究集会
  • Place of Presentation: 淡路夢舞台国際会議場(兵庫県)
  • Year and Date: 2007-07-02
• [Presentation] Isolation of an endoplasimic reticulum protein as a candidate ligand for Draper, a Drosophila phagocytosis receptor (2007)
  • Author(s): Takayuki Kuraishi
  • Organizer: 第8回日本ショウジョウバエ研究集会
  • Place of Presentation: 淡路市
  • Year and Date: 2007-07-02
• [Presentation] Isolation of putative ligand for Draper, Drosophila homologue of C. elegans CED-1, responsible for apoptotic cell clearance and axonal pruning (2007)
  • Author(s): Y. Nakanishi
  • Organizer: Gordon Conference Apoptotic Cell Recognition and Clearance
  • Place of Presentation: Bates College(米国)
  • Year and Date: 2007-06-19
• [Presentation] Isolation of putative Iigand for Draper, Drosophila homologue of C. elegans CED-1, responsiible for apoptotic cell clearance and axonal pruning (2007)
  • Author(s): Yoshinobu Nakanishi
  • Organizer: Gordon Conference Apoptotic Cell Recognition and Clearance
  • Place of Presentation: Lewiston,Maine,USA
  • Year and Date: 2007-06-19
• [Book] 実験医学増刊号、細胞死による多様な生体制御、第3章の2. アポトーシス依存的な細胞貪食による生体恒常性の維持 (2010)
  • Author(s): 白土明子、中西義信
  • Publisher: 羊土社
• [Book] arget Pattern Recognition in Innate Immunity, U. Kishore (編), 第9章(Pattern recognition in phagocytic clearance of altered self) (2009)
  • Author(s): Nakanishi, Y., Henson, P. M.
  • Publisher: Landes Bioscience
• [Remarks]
  • URL: http://www.p.kanazawa-u.ac.jp/~seibutu/
• [Remarks]
  • URL: http://www.p.kanazawa-u.acjp/~seibutu/
• [Remarks] 該当せず