| Project/Area Number |
23592526
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Kyoto University |
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Principal Investigator |
TATEYA ICHIRO 京都大学, 医学(系)研究科(研究院), 講師 (20526363)
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| Co-Investigator(Kenkyū-buntansha) |
HIRANO Shigeru 京都大学, 大学院医学研究科, 講師 (10303827)
MUTO Manabu 京都大学, 大学院医学研究科, 教授 (40360698)
KITMURA Morimasa 京都大学, 大学院医学研究科, 助教 (60543262)
KADA Shinpei 独立行政法人国立病院機構京都医療センター, 臨床研究センター, 研究員 (70543263)
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| Co-Investigator(Renkei-kenkyūsha) |
SETOU Mitsutoshi 浜松医科大学, 教授 (20302664)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 耳鼻咽喉科学 / 頭頸部外科学 / 表在癌 / 質量顕微鏡 / アラキドン酸 / 上皮下浸潤 / 質量分析 / 咽頭 / 下咽頭癌 / 発現プロファイリング / イメージングマススペクトロメトリー / 免疫染色 |
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| Research Abstract |
The number of cases of superficial-type pharyngeal squamous cell carcinoma (STPSCC) has increased recently. An exploration of the biological characteristics of STPSCC could uncover the invasion mechanism of this carcinoma. Phosphatidylcholine (PC) is the most abundant component of cell lipid bilayers. However, the distribution of PC in early invasive carcinomas has not been determined. Imaging mass spectrometry (IMS) can be used to visualize the distribution of biomolecules on a tissue section, and is especially suitable for phospholipids. We conducted an IMS analysis to elucidate the distribution of PC species in STPSCC tissues from five patients. We determined by tandem mass spectrometry that three kinds of PC species with arachidonic acid, were expressed at significantly higher levels in the submucosal invasive region than in the superficial region of STPSCC. These distributional differences could be associated with the mechanism of hypopharyngeal carcinoma invasion.
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