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Lymphoangiogenic interaction of tumor and stromal environment in oral cancer xenograft mouse model

Research Project

Project/Area Number 25462935
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Pathobiological dentistry/Dental radiology
Research InstitutionThe Nippon Dental University

Principal Investigator

Soeno Yuuichi  日本歯科大学, 生命歯学部, 准教授 (70350139)

Co-Investigator(Kenkyū-buntansha) TAYA Yuji  日本歯科大学, 生命歯学部, 准教授 (30197587)
SATO Kaori  日本歯科大学, 生命歯学部, 講師 (90287772)
SHIMAZU Yoshihito  麻布大学, 生命・環境科学部, 准教授 (10297947)
AOBA Takaaki  日本歯科大学, 生命歯学部, 教授 (30028807)
FUJITA Kazuya  日本歯科大学, 生命歯学部, 助教 (70549055)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords腫瘍 / 扁平上皮癌 / リンパ節転移 / リンパ管新生 / 遺伝子発現 / 移植モデル / 同所移植モデル / 癌微小環境 / 血管新生 / 歯学 / 病理学
Outline of Final Research Achievements

We developed a xenograft mouse model to explore the heterogeneity of the tumor microenvironment. We transplanted five oral carcinoma cell lines into the tongues of nude mice and determined tongue tumor growth and micrometastatic dissemination. All cell lines tested were tumorigenic in mouse tongue. The metastatic lesion-derived carcinoma cell lines (OSC19, OSC20, and HSC2) yielded a 100% nodal metastasis rate, whereas the primary tumor-derived cell lines (KOSC2 and HO-1-u-1) showed < 40% metastatic potential. Immunohistochemistry showed that the individual cell lines gave rise to heterogeneous tumor architecture and phenotypes. Notably, OSC19 and OSC20 cells shared similar aggressive tumorigenicity in both the tongue and lymph node environments but displayed markedly diverse immunophenotypes and gene expression profiles. The results support that tumorigenicity and tumor architecture in the host tongue environment depend on the origin and properties of the carcinoma cell lines.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (19 results)

All 2016 2015 2014 2013 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (11 results) (of which Int'l Joint Research: 1 results) Book (2 results) Remarks (3 results)

  • [Journal Article] Heterogeneous tumor stromal microenvironments of oral squamous cell carcinoma cells in tongue and nodal metastatic lesions in a xenograft mouse model2015

    • Author(s)
      Shirako Y, Taya Y, Sato K, Chiba T, Imai K, Shimazu Y, Aoba T, Soeno Y
    • Journal Title

      J Oral Pathol. Med

      Volume: 44 Issue: 9 Pages: 656-668

    • DOI

      10.1111/jop.12318

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 3) Three-dimensional reconstruction of oral tongue squamous cell carcinoma at invasion front.2013

    • Author(s)
      Kudo T, Shimazu Y, Yagishita H, Izumo T, Soeno Y, Sato K, Taya Y, Aoba T.
    • Journal Title

      Int J Dent.

      Volume: On line Pages: 1-11

    • DOI

      10.1155/2013/482765

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Generation of a mouse model with down-regulated U50 snoRNA (SNORD50) expression and its organ-specific phenotypic modulation2013

    • Author(s)
      Soeno Y, Fujita K, Kudo T, Asagiri M, Kakuta S, Taya Y, Shimazu Y, Sato K, Tanaka-Fujita R, Kubo S, Iwakura Y, Nakamura Y, Mori S, and Aoba T
    • Journal Title

      PLoS ONE

      Volume: 8 Issue: 8 Pages: e72105-e72105

    • DOI

      10.1371/journal.pone.0072105

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Stromal configuration by human carcinoma transplantation in mouse tongue2016

    • Author(s)
      Soeno Y, Shirako Y, Taya Y, Shimazu Y, Sato K
    • Organizer
      American Association for Dental Research
    • Place of Presentation
      Los Angeles Convention Center (Los Angeles, CA, USA)
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マウス舌移植モデルにおけるヒト口腔扁平上皮癌細胞の浸潤・転移形質と癌微小環境2015

    • Author(s)
      白子要一,佐藤かおり,田谷雄二,青葉孝昭,島津徳人,添野雄一
    • Organizer
      歯科基礎医学会
    • Place of Presentation
      朱鷺メッセ(新潟県新潟市中央区)
    • Year and Date
      2015-09-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] 癌微小環境と非癌性病変にみられる脈管間質の多次元形態解析2015

    • Author(s)
      添野雄一,白子要一,島津徳人,田谷雄二,佐藤かおり,柳下寿郎,青葉孝昭
    • Organizer
      日本病理学会
    • Place of Presentation
      名古屋国際会議場(愛知県名古屋市熱田区)
    • Year and Date
      2015-04-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] 扁平上皮癌の同所移植モデルにおける原発-リンパ節転移環境での脈管間質形成の比較2014

    • Author(s)
      添野雄一,白子要一,島津徳人,田谷雄二,佐藤かおり,青葉孝昭
    • Organizer
      日本病理学会
    • Place of Presentation
      広島国際会議場(広島県広島市中区)
    • Year and Date
      2014-04-26
    • Related Report
      2014 Research-status Report
  • [Presentation] 癌微小環境における脈管新生の3次元形態解析:腫瘍-宿主境界での脈管チャネルの空間分布2014

    • Author(s)
      白子要一,添野雄一,島津徳人,田谷雄二,佐藤かおり,青葉孝昭
    • Organizer
      日本病理学会
    • Place of Presentation
      広島国際会議場(広島県広島市中区)
    • Year and Date
      2014-04-26
    • Related Report
      2014 Research-status Report
  • [Presentation] Intraepithelial 3D-architectural analysis of tongue premalignant-cancerous progression2014

    • Author(s)
      Henmi T., Shimazu Y., Soeno Y., Yagishita H., Okabe S., Taya Y., Shimazu Y., Sato K., Aoba T.
    • Organizer
      43rd Annual Meeting & Exhibition of the AADR
    • Place of Presentation
      CHARLOTTE, N.C. USA
    • Related Report
      2013 Research-status Report
  • [Presentation] Three-dimensionalVisualization of Tumor-host Interface in Xenograft Mouse Model2014

    • Author(s)
      Soeno Y., Shirako Y., Fujita K., Taya Y., Shimazu Y., Sato K., Chiba T., Imai K., Aoba T.
    • Organizer
      43rd Annual Meeting & Exhibition of the AADR
    • Place of Presentation
      CHARLOTTE, N.C. USA
    • Related Report
      2013 Research-status Report
  • [Presentation] 腫瘍浸潤様式の3次元形態解析:扁平上皮癌における微小浸潤胞巣の成立機序2013

    • Author(s)
      添野雄一、島津徳人、田谷雄二、中右かよ、白子要一、藤田和也、佐藤かおり、柳下寿郎、青葉孝昭
    • Organizer
      第102回日本病理学会総会
    • Place of Presentation
      北海道 ロイトン札幌、さっぽろ芸文館
    • Related Report
      2013 Research-status Report
  • [Presentation] 腫瘍微小環境の3次元形態学:Virtual Reality観察の導入2013

    • Author(s)
      島津徳人、添野雄一、白子要一、藤田和也、田谷雄二、中右かよ、佐藤かおり、青葉孝昭
    • Organizer
      第102回日本病理学会総会
    • Place of Presentation
      北海道 ロイトン札幌、さっぽろ芸文館
    • Related Report
      2013 Research-status Report
  • [Presentation] 腫瘍内間質構築と脈管新生誘導:ヒト口腔癌マウス舌移植モデルにおける形態解析2013

    • Author(s)
      白子要一、添野雄一、藤田和也、島津徳人、田谷雄二、佐藤かおり、青葉孝昭
    • Organizer
      第102回日本病理学会総会
    • Place of Presentation
      北海道 ロイトン札幌、さっぽろ芸文館
    • Related Report
      2013 Research-status Report
  • [Presentation] 脈管構造の組織立体構築とVirtual Relity観察2013

    • Author(s)
      島津徳人、田谷雄二、添野雄一、白子要一、藤田和也、佐藤かおり、青葉孝昭
    • Organizer
      第55回歯科基礎医学会学術大会ならびに総会
    • Place of Presentation
      岡山県 岡山コンベンションセンター
    • Related Report
      2013 Research-status Report
  • [Book] 歯学生のための最新・病態病理学入門2014

    • Author(s)
      青葉孝昭 監修、佐藤かおり 編集、田谷雄二・添野雄一 分担執筆
    • Total Pages
      177
    • Publisher
      株式会社キタ・メディア
    • Related Report
      2013 Research-status Report
  • [Book] 今すぐ始める組織立体構築―ImageJ/Fijiの活用プロトコール―2013

    • Author(s)
      島津徳人 編、青葉孝昭 監修、田谷雄二・添野雄一・佐藤かおり 執筆
    • Total Pages
      22
    • Publisher
      日本ビジュアルサイエンス株式会社
    • Related Report
      2013 Research-status Report
  • [Remarks] 日本歯科大学生命歯学部病理学講座HP

    • URL

      http://www.ndu.ac.jp/~pathhome/

    • Related Report
      2015 Annual Research Report
  • [Remarks] 日本歯科大学病理学講座ホームページ

    • URL

      http://www.ndu.ac.jp/~pathhome/

    • Related Report
      2014 Research-status Report
  • [Remarks] 日本歯科大学病理学講座HP

    • URL

      http://www.ndu.ac.jp/~pathhome/

    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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