Generation and application of transgenic mice for wide-field and multi-color calcium imaging
Project/Area Number |
18K19493
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Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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Allocation Type | Multi-year Fund |
Review Section |
Medium-sized Section 51:Brain sciences and related fields
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Research Institution | The University of Tokyo |
Principal Investigator |
Sakamoto Masayuki 東京大学, 大学院医学系研究科(医学部), 助教 (00777865)
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Project Period (FY) |
2018-06-29 – 2020-03-31
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Project Status |
Completed (Fiscal Year 2019)
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Budget Amount *help |
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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Keywords | カルシウムイメージング / 遺伝子改変マウス / 2光子励起顕微鏡 / 多色イメージング / 遺伝的改変マウス / 2光子励起顕微鏡 |
Outline of Final Research Achievements |
To achieve wide-field and multi-color calcium imaging with high-speed and high-sensitive calcium indicators, we generated and evaluated novel transgenic mice that express red calcium indicator and green calcium indicator in excitatory neurons inhibitory neurons, respectively. As a result, we succeeded to establish new transgenic lines that can detect fluorescent signals in response to spontaneous and sensory-evoked activity in vivo with two-photon microscopy.
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Academic Significance and Societal Importance of the Research Achievements |
高次脳機能を制御する神経ネットワークを理解するためには、一細胞レベルの解像度を保ちつつ、広域から多数のニューロンの活動を同時に計測する必要がある。本研究で樹立した新規遺伝子改変マウスを応用することで、興奮性ニューロンと抑制性ニューロン活動を計測することが可能となった。これらマウスを用いることで、興奮性ニューロンと抑制性ニューロンが織りなす高度に制御されたネットワークダイナミクスについて理解できるものと期待される。
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Report
(3 results)
Research Products
(6 results)
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[Journal Article] Rational engineering of XCaMPs, a multicolor GECI suite for in vivo imaging of complex brain circuit dynamics.2019
Author(s)
Inoue M, Takeuchi A, Manita S, Horigane S-i, Sakamoto M, Kawakami R, Yamaguchi K, Otomo K, Yokoyama H, Kim R, Yokoyama T, Takemoto-Kimura S, Abe M, Okamura M, Kondo Y, Quirin S, Ramakrishnan C, Imamura T, Sakimura K, Nemoto T, Kano M, Fujii H, Deisseroth K, Kitamura K, Bito H.
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Journal Title
Cell
Volume: 177
Issue: 5
Pages: 1346-1360
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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