| Project/Area Number |
21790960
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Infectious disease medicine
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| Research Institution | Nagasaki University |
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Principal Investigator |
UECHI Genichiro Nagasaki University, 熱帯医学研究所, 助教 (10420647)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 感染症治療学 / H5N1 / リボソームディスプレイ法 / ScFv抗体 / パニング / 無細胞発現系 / 抗体 |
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| Research Abstract |
The purpose of this study is isolating H5N1 neutralizing human that recognize H5N1 antigen specifically and characterize its neutralizing activity and determine epitope.
After three round of panning, concentrated scFv genes were ligated into E.coli expression vector and total 1200 colonies were picked up and scFv expression level was analyzed by ELISA. Total 12 of 30 scFvs showed binding activity against recombinant Hemagglutinin protein of H5N1. The twelve scFvs were cultured in large scale and obtained about 6mg from 1L culture scale. Total 1 of 12 sample showed neutralizing activity against A/Vietnam/31244/07 strain at the concentration of 62μg/ml (2.5nM). This scFv antibody recognized recombinant HA specifically, now escape mutant analysis is on going.
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