Re-direction of the differentiation status by a novel protein transduction system LENA

• Project/Area Number: 24310146
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Medical genome science
• Research Institution: National Hospital Organization Nagoya Medical Center (2014); Osaka Prefectural Institute of Public Health (2012-2013)
• Principal Investigator: KOMANO Atsushi 独立行政法人国立病院機構(名古屋医療センター臨床研究センター), その他部局等, その他 (60356251)
• Co-Investigator(Kenkyū-buntansha): TAKEDA Satoshi 国立感染症研究所, エイズ研究センター, 研究員 (50396959)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥20,280,000 (Direct Cost: ¥15,600,000、Indirect Cost: ¥4,680,000); Fiscal Year 2014: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000); Fiscal Year 2013: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2012: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
• Keywords: 移植•再生医療 / バイオテクノロジー / レンチウイルス様ナノ粒子 / 脱分化法 / ナノバイオ / 再生医療 / 移植・再生医療

Outline of Final Research Achievements

For the establishment of safe re-direction protocol, we evaluated the protein delivery system LENA. We have shown that a transcription factor. Oct3/4 is successfully delivered to human cells via LENA. However, we found that the system have a room to improve because the biological function of Oct3/4 is detected weakly. We designed the second generation LENA to achieve the goal of this study.

Research Products

• [Journal Article] Impact of Genotype-specific Herd Immunity on the Circulatory Dynamism of Norovirus: A 10-year Longitudinal Study of Viral Acute Gastroenteritis (2015)
  • Author(s): Sakon N, Yamazaki K, Nakata K, Kanbayashi D, Yoda T, Mantani M, Kase T, Takahashi K, *Komano J.
  • Journal Title: Journal of Infectious Diseases Volume: 211 Pages: 879-888
  • DOI: 10.1093/infdis/jiu496
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The reply. Pitfalls of National Surveillance Systems for Vaccine-associated Measles (2014)
  • Author(s): Kurata T, Kanbayashi D, Komano J*, Kase T, Takahashi K.
  • Journal Title: The American Journal of Medicine Volume: 127 Pages: 19-19
  • DOI: 10.1016/j.amjmed.2014.04.027
  • Peer Reviewed / Open Access
• [Journal Article] Novel role of HSP40/DNAJ in the regulation of HIV-1 replication (2013)
  • Author(s): Urano E, Morikawa Y, Komano J
  • Journal Title: JAIDS Volume: (In press)
  • Peer Reviewed
• [Journal Article] A novel therapeutic molecule against HTLV-1 infection targeting provirus. (2013)
  • Author(s): Tanaka A, Takeda S, Kariya R, Matsuda K, Urano E, Okada S, Komano J
  • Journal Title: Leukemia Volume: (In press)
  • DOI: 10.1038/leu.2013.46
  • Peer Reviewed
• [Journal Article] Prevalence and epidemiological traits of HIV infections in populations with high risk behaviors as revealed by genetic analysis of HBV. (2013)
  • Author(s): Kojima Y, Kawahata T, Mori H, Furubayashi K, Taniguchi Y, Iwasa A, Taniguchi K, Kimura H, Komano J
  • Journal Title: Epidemiology and Infection. Pages: 1-8
  • Peer Reviewed
• [Journal Article] Elicitation of both anti HIV-1 Env humoral and cellular immunities by replicating vaccinia prime Sendai virus boost regimen and boosting by CD40Lm. (2012)
  • Author(s): Zhang X
  • Journal Title: PLoS One Volume: 7 Pages: 1-11
  • DOI: 10.1371/journal.pone.0051633
  • Peer Reviewed
• [Journal Article] Toll-like receptor (TLR) 3 as a potential sensor of retroviral infection in human cells. (2012)
  • Author(s): Miyauchi K, Urano E, Takeda S, Murakami T, Okada Y, Cheng K, Yin H, Kubo M, Komano J
  • Journal Title: Biochem Biophys Res Commun. Volume: 12 Pages: 109-116
  • DOI: 10.1016/j.bbrc.2012.06.148
  • Peer Reviewed
• [Journal Article] Regulation of Cyclin T1 expression and function by an alternative splice variant that skips exon 7 and contains a premature termination codon. (2012)
  • Author(s): Urano E, Miyauchi K, Ichikawa R, Futahashi Y, Komano J
  • Journal Title: Gene. Volume: 505 Pages: 1-8
  • DOI: 10.1016/j.gene.2012.06.006
  • Peer Reviewed
• [Journal Article] Neutrophil extracellular traps mediate a host defense response to human immunod eficiency virus-1 (2012)
  • Author(s): Saitoh T, Komano J, Saitoh Y, Misawa T, Takahama M, Kozaki T, Uehata T, Iwasaki H, Omori H, Yamaoka S, Yamamoto N, Akira S.
  • Journal Title: Cell Host Microbe. Volume: 12 Pages: 109-116
  • Peer Reviewed
• [Journal Article] Structural modulation study of inhibitory compounds for RNase H activity of HIV-1 reverse transcriptase. (2012)
  • Author(s): Saitoh T, Komano J, Saitoh Y, Misawa T, Takahama M, Kozaki T, Uehata T, Iawasaki H. Omori H, Yamaoka S, Yamamoto N, Akira S.
  • Journal Title: Chem Pharm. Bull (Tokyo). Volume: 12(1) Pages: 109-116
  • DOI: 10.1016/j.chom.2012.05.015
  • Peer Reviewed
• [Journal Article] Evaluation of a synthetic C34 trimer of HIV-1 gp41 as AIDS vaccines. (2012)
  • Author(s): Hashimoto, C., Nomura, W., Ohya, A., Urano, E., Miyauchi, K., Narumi, T., Aikawa, H., Komano, J.A., Yamamoto, N., Tamamura, H.
  • Journal Title: Bioorg Med Chem. Volume: (in press) Pages: 3287-3291
  • DOI: 10.1016/j.bmc.2012.03.050
  • Peer Reviewed
• [Journal Article] Therapeutic potential of HIV protase-activable CASP3. (2012)
  • Author(s): Miyauchi K, Urano E, Takizawa M, Ichikawa R, Komano J
  • Journal Title: Scientific Reports. Volume: 2 Pages: 359-359
  • DOI: 10.1038/srep00359
  • Peer Reviewed
• [Journal Article] A Synthetic C34 Trimer of HIV-1 gp41 Shows Significant Increase of In hibition Potency. (2012)
  • Author(s): Nomura W, Hashimoto C, Ohya A, Miyauchi K, Urano E, Tanaka T, Narumi T, Nakahara T, Komano J, Yamamoto N, Tamamura H
  • Journal Title: ChemMedChem. Volume: 7 Pages: 546-546
  • Peer Reviewed
• [Presentation] 先天性風疹症候群の追跡調査の現状と出生時診断の留意点について. (2015)
  • Author(s): 上林大起, 倉田貴子, 福村和美, 畑中己穂, 田邊雅章, 松本治子, 駒野 淳, 加瀬哲男, 高橋和郎, 五十嵐愛, 北島博之
  • Organizer: 第26回日本臨床微生物学会総会・学術集会
  • Place of Presentation: 東京
  • Year and Date: 2015-01-31 – 2015-02-01
• [Presentation] 急速な病期進行をみた感染初期例群に共通して検出された新規変異HIV-1の流行実態. (2014)
  • Author(s): 森 治代, 小島洋子, 川畑拓也, 駒野 淳.
  • Organizer: 第28回日本エイズ学会学術集会・総会
  • Place of Presentation: 大阪
  • Year and Date: 2014-12-03 – 2014-12-05
• [Presentation] βグルコセレブロシダーゼが持つエンテロウイルス 71 感染症の分子標的治療薬としての潜在性. (2014)
  • Author(s): 中田恵子, 駒野 淳.
  • Organizer: 第62回日本ウイルス学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-10 – 2014-11-12
• [Presentation] 生物発光を利用した風疹ウイルス検出系の実験室診断への応用~流行要因解明に向けて~ (2014)
  • Author(s): 上林大起, 倉田貴子, 駒野 淳
  • Organizer: 第73回日本公衆衛生学会総会
  • Place of Presentation: 宇都宮.
  • Year and Date: 2014-11-05 – 2014-11-06
• [Presentation] Measles virus as a potential oncolytic virotherapy against B cell lymphomas. (2014)
  • Author(s): 1)武田 哲, 上林大起, 倉田貴子, 吉山 裕樹, 駒野 淳.
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-25 – 2014-09-26
• [Presentation] Non-coding RNA(7SK)の転写抑制機能を模倣したRNAモチーフの探索 (2012)
  • Author(s): 山吉麻子, 岸本恭介, 林里依, 駒野淳, 小柳義夫, 小堀哲生, 村上章
  • Organizer: 第58回高分子研究発表会
  • Place of Presentation: 神戸
  • Year and Date: 2012-07-13
• [Presentation] 安全な細胞内免疫を可能にする治療分子の開発に関する研究 (2012)
  • Author(s): 駒野淳, 浦野恵美子, 宮内浩典, 市川玲子, 滝沢真理
  • Organizer: 第26回近畿エイズ研究会・学術集会
  • Place of Presentation: 神戸
  • Year and Date: 2012-01-07
• [Presentation] Toll-like receptor(TLR)3 as a potential sensor of retroviral nfection in human cell (2012)
  • Author(s): K Miyauchi, E Urano, S Takeda, T Murakami, Y Okada, K Cheng, H Yin, M Kubo, J Komano
  • Organizer: CSHL meeting on Retroviruses
  • Place of Presentation: Cold Spring Harbor, NY, USA
• [Presentation] P-TEFb複合体機能調節因子群によるHTLV-1転写制御メカニズムの解析 (2012)
  • Author(s): 浦野恵美子, 木村雄一, 駒野淳
  • Organizer: 第5回HTLV-1研究会
  • Place of Presentation: 東京
• [Presentation] Proliferative inhibition of ATL-derived and HTLV-1-transformed human Tcells by an artificial restriction (2012)
  • Author(s): 駒野淳, 田中淳, 武田哲
  • Organizer: 第5回HTLV-1研究会
  • Place of Presentation: 東京
• [Presentation] A novel therapeutic molecule that irreversibly disrupts the HTLV-1 provirus (2012)
  • Author(s): J Komano, A Tanaka, S Takeda, R Ichikawa, E Urano
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
• [Presentation] 再生医療によるHIV/AIDS遺伝子細胞治療法の開発に向けた技術基盤の構築 (2012)
  • Author(s): 武田哲, 駒野淳
  • Organizer: 第59回日本ウィルス学会学術集会
  • Place of Presentation: 大阪
• [Presentation] HTLV-1プロウイルスを物理的に傷害する人工酵素の開発 (2012)
  • Author(s): 駒野淳, 田中淳, 武田哲
  • Organizer: 第59回日本ウィルス学会学術集会
  • Place of Presentation: 大阪
• [Presentation] プロウイルスの物理的障害によるHTLV-1感染細胞増殖の抑制 (2012)
  • Author(s): 武田哲, 田中淳, 駒野淳
  • Organizer: 第59回日本ウイルス学会学術集会
  • Place of Presentation: 大阪
• [Presentation] HBV感染者における新規Ae/GリコンビナントHBVの解析 (2012)
  • Author(s): 小島洋子, 川畑拓也, 森治代, 駒野淳, 谷口恭, 井戸田一朗
  • Organizer: 第59回日本ウイルス学会学術集会
  • Place of Presentation: 大阪
• [Presentation] ヒト化マウスを用いたEBウイルス関連リンパ増殖性疾患に対する免疫細胞治療のモデル実験 (2012)
  • Author(s): 松田剛, 今留謙一, 矢島美彩子, 落合央, 望月雅司, 川野布由子, 山田千尋、今井由美, 濱崎霞, 浅田恵理子, 原口摩耶, 千葉祐規乃, 清水則夫, 駒野淳、, 山本直樹, 藤原成悦
  • Organizer: 第59回日本ウイルス学会学術集会
  • Place of Presentation: 大阪
• [Presentation] DNA J/HSP40 Co-chaperone familyによるHIV-1複製抑制メカニズムの解析 (2012)
  • Author(s): 駒野淳, 浦野恵美子
  • Organizer: 第25回日本エイズ学会学術集会・総会
  • Place of Presentation: 横浜
• [Presentation] 弱毒ワクシニアプライム、センダイウイルスブーストエイズワクチン法の免疫評価およびhCD40Lmアジュバント効果の検討 (2012)
  • Author(s): 張険峰, 祖父江友芳, 一色真央, 牧野俊, 井上誠, 加藤和則, 塩田達夫, 大橋貴, 駒野淳, 花房秀次, 志田壽利
  • Organizer: 第25回日本エイズ学会学術集会・総会
  • Place of Presentation: 横浜
• [Presentation] ペプチドミメティック型インテグラーゼ阻害剤の構造活性相関研究 (2012)
  • Author(s): 相川春夫, 松本大地, 野末愛美, 浦野恵美子, Mathieu Metifiot, Maddali Kasthuraiah, 野村渉, 鳴海哲夫, 駒野淳, 村上努, Pommier Yves, 山本直樹, 玉村啓和
  • Organizer: 第25回日本エイズ学会学術集会・総会
  • Place of Presentation: 横浜
• [Presentation] Therapeutic potential of an artificial endonuclease against HTLV-1 infection (2012)
  • Author(s): 駒野淳, 武田哲, 田中淳
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Patent(Industrial Property Rights)] HIV立体構造認識抗体誘導ペプチド (2012)
  • Inventor(s): 玉村啓和, 鳴海哲夫, 野村渉, 橋木知恵, 駒野淳, 宮内浩典,
  • Industrial Property Rights Holder: 玉村啓和, 鳴海哲夫, 野村渉, 橋木知恵, 駒野淳, 宮内浩典,
  • Filing Date: 2012-04-03
  • Overseas