• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

染色体機能装置のダイナミクスとアルゴリズム

Research Project

Project/Area Number 11239101
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionNara Institute of Science and Technology

Principal Investigator

吉川 寛  奈良先端科学技術大学院大学, 名誉教授 (70019876)

Co-Investigator(Kenkyū-buntansha) 松浦 彰  長寿医療研究センター, 老年病研究部, 室長 (10272692)
菊池 韶彦 (菊地 詔彦)  名古屋大学, 医学部, 教授 (40283428)
太田 邦史  理化学研究所, 遺伝生化学教室, 主任研究員 (90211789)
白髭 克彦  東京工業大学, バイオ研究基盤支援総合センター, 助教授 (90273854)
平岡 泰  通信総合研究所, 関西先端研究センター, 主任研究員
舛本 寛 (桝本 寛)  名古屋大学, 大学院・理学研究科, 講師 (70229384)
水野 重樹  日本大学, 生物資源科学部, 教授 (90112903)
石川 冬木  京都大学, 大学院・理学研究科, 教授 (30184493)
山本 正幸  東京大学, 大学院・理学研究科, 教授 (40114706)
平賀 荘太  熊本大学, 医学部, 教授 (40027321)
柳田 充弘  京都大学, 大学院・理学研究科, 教授 (80025428)
Project Period (FY) 1999 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥281,400,000 (Direct Cost: ¥281,400,000)
Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2003: ¥34,300,000 (Direct Cost: ¥34,300,000)
Fiscal Year 2002: ¥52,000,000 (Direct Cost: ¥52,000,000)
Fiscal Year 2001: ¥60,900,000 (Direct Cost: ¥60,900,000)
Fiscal Year 2000: ¥56,700,000 (Direct Cost: ¥56,700,000)
Fiscal Year 1999: ¥76,000,000 (Direct Cost: ¥76,000,000)
Keywordsクロマチン構造 / 染色体動態 / 分配 / 組換え / 複製 / 修復 / 転写 / 染色体構築 / 複製チェックポイント / 染色体分配 / 減数分裂 / DNAチップ / クロマチンリモデリング / 染色体構造 / セントロメア / テロメア / 複製開始点 / ヘテロクロマチン / ユークロマチン / 染色体 / チェックポイント / 老化 / 染色体機能 / 染色体高次構造 / 人工染色体 / 核 / 姉妹染色体接着 / 細胞周期
Research Abstract

最終年度のまとめをかねて、班員全員で公開シンポジウム「生命を育むプラットフォーム染色体」を開催した(10月24日、東京ガーデンパレス)。来聴者数は約150人を超え、質疑応答も非常に活発に行われたところから、ある程度のこの分野の啓蒙に寄与することが出来た。以下に簡単なプログラムを付す。
染色体と老化(座長:白髭克彦)
石川冬木 テロメア維持の仕組みと細胞寿命についての研究成果
松浦彰 テロメア維持の仕組みと細胞寿命についての研究成果
菊池韶彦 トポイソメラーゼやSgs1などの研究成果
染色体の複製・分配(座長:松浦彰)
白髭克彦 複製因子などのChIP chipなどの研究成果
奥村克純 複製部位やヘテロクロマチンの視覚化についての研究成果
染色体の変化〜クロマチン・核構造のダイナミクス(座長:菊池韶彦)
筒井研 核マトリクス・トポイソメラーゼについての研究成果
土屋英子 クロマチン再編成因子についての研究成果
染色体と生命の伝承〜減数分裂と組換え(座長:土屋英子)
平岡泰 減数分裂における染色体のダイナミックな挙動などについての研究成果
太田邦史 減数分裂期組換えの仕組みとその応用
また、班員全員の業績をまとめた業績集を編集し、5年間のまとめとした。

Report

(6 results)
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (35 results)

All 2004 2003 2001 Other

All Journal Article (8 results) Publications (27 results)

  • [Journal Article] CENP-B interacts with CENP-C domains containing Mif2 regions responsible for centromere localization.2004

    • Author(s)
      N.Suzuki, M.Nakano, N.Nozaki, S.Egashira, T.Okazaki, H.Masumoto
    • Journal Title

      J.Biological Chem 279

      Pages: 5934-5946

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Reciprocal association of the budding yeast ATM-related proteins Tel1 and Mec1 with telomeres in vivo.2004

    • Author(s)
      H.Takata, Y.Kanoh, N.Gunge, K.Shirahige, A.Matsuura
    • Journal Title

      Mol.Cell. 14

      Pages: 515-522

    • Related Report
      2004 Annual Research Report
  • [Journal Article] S-phase checkpoint proteins Tof1 and Mrc1 form a stable replication pausing complex.2004

    • Author(s)
      Y.Katou, Y.Kanoh, M.Bando, H.Noguchi, H.Tanaka, T.Ashikari, K.Sugimoto, K.Shirahige
    • Journal Title

      Nature 424

      Pages: 1078-1083

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Roles of histone acetylation and chromatin remodeling factor in a meiotic recombination hospot2004

    • Author(s)
      T.Yamada, K.Mizuno, K.Hirota, N.Kon, W.P.Wahls, E.Hartsuiker, H.Murofushi, T.Shibata, K.Ohta
    • Journal Title

      EMBO J. 23

      Pages: 1792-1803

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Localized expression of amphiphysin Ir, a retina-specific variant of amphiphysin I, in the ribbon synapse and its functional implication2004

    • Author(s)
      O.Hosoya, K.Tsutsui, K.Tsutsui
    • Journal Title

      Eur.J.Neurosci 19

      Pages: 2179-2187

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Functional compatibility between isoform α and β of type II DNA topoisomerase2004

    • Author(s)
      A.Sakaguchi, A.Kikuchi
    • Journal Title

      J Cell Sci 117

      Pages: 1047-1054

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Cohesin relocation from sites of chromosomal loading to places of convergent transcriptions.2003

    • Author(s)
      A.Lengronne, Y.Katou, S.Mori, S.Yokobayashi, G.Kelly, T.Itoh, Y.Watanabe, K.Shirahige^*, F.Uhlrnann^* (^*equally contributed author)
    • Journal Title

      Nature 430

      Pages: 573-578

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Mapping Sites Where Replication, Initiates in Mammalilan Cells Using DNA Fibers.2001

    • Author(s)
      S.Takebayashi, E.M.M.Manders, H.Kimura, H.Taguchi, K.Okumura
    • Journal Title

      Exp.Cell Res. 271(2)

      Pages: 263-268

    • Related Report
      2004 Annual Research Report
  • [Publications] Shirahige K: "S-phase checkpoint proteins Tof1 and Mrc1 form a stable replication-pausing complex."nature. 424. 1078-1083 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ohta K.: "Roles of histone acetylation and chromatin remodeling factor in meiotic recombination hotspot"EMBO Journal. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kikuchi A.: "Genome-wide expression analysis of NAP1 in Saccharomyces cerevisiae."Biochem Biophys Res Commun.. 306. 5-9 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Masumoto H.: "Epigenetic assembly of centromeric chromatin at ectopic alpha-satellite sites on human chromosomes."J Cell Sci.. 116. 4021-4034 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hiraoka Y: "Monopolar spindle attachment of sister chromatids is ensured by two distinct mechanisms at the first meiotic division in fission yeast."EMBO J.. 22. 2284-2296 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Matsuura A: "Reciprocal association of the budding yeast ATM-related proteins Tell and Mec1 with telomeres in vivo."Mol.Cell. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] A.Pecina, K.Smith, C.Mezard, H.Murakami, K.Ohta, A.Nicolas: "Target stimulation of meiotic recombination"Cell. 111. 173-184 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] J.Ohzeki, M.Nakano, T.Okada, H.Masumoto: "CENP-B box is required for de novo centromere chromatin assembly on human alphoid DNA"J. Cell Biol.. 159. 765-775 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Koyama, M.Itoh, K.Miyahara, E.Tsuchiya: "Abundance of the RSC nucleosome-remodeling complex is important for the cells to tolerate DNA damage in Saccharomyces cerevisiae"FEBS Letters. 531. 215-221 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Watanabe, J.Morishita, K.Umezu, K.Shirahige, H.Maki: "Involvement of RAD9-dependent damage checkpoint control in arrest of cell cycle, induction of cell death, and chromosome instability caused by defects in origin recognition complex in Saccharomyces cerevisiae"Eukaryot Cell.. 1. 200-212

    • Related Report
      2002 Annual Research Report
  • [Publications] Chikashige, Y, Hiraoka, Y: "Telomere binding of the Rap1 protein is required for meiosis in fission Yeast"Curr Biology. 11. 1618-1623 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Mizuno Ki K, Hasemi T, Ubukata T, Yamada T, Lehmann E, Kohli J, Watanabe Y, Iino Y, Yamamoto M, Fox ME, Smith GR, Murofushi H, Shibata T, Ohta K.: "Counteracting Regulation of Chromatin Remodeling at a Fission Yeast cAMP Responsive Element-Related Recombination Hotspot by Stress-Activated Protein Kinase, cAMP-Dependent Kinase and Meiosis Regulators"Genetics. 159. 1467-1478 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] J.M.Kim, H.Yoshikawa, K.Shirahige: "A member of the YER057c/yjgf/Uk114 family links isoleucine biosynthesis and intact mitochondria maintenance in Saccharomyces cerevisiae"Genes to Cells. 6. 507-517 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takebayashi SI, Manders EM, Kimura H, Taguchi H, Okumura K.: "Mapping sites where replication initiates in mammalian cells using DNA fibers"Exp Cell Res.. 271. 263-268 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tadokoro R, Fujita M, Miura H, Shirahige K, Yoshikawa H, Tsurimoto T, Obuse C: "Scheduled conversion of replication complex architecture at replication origins of S. cerevisiae during the cell cycle"J Biol Chem.. (In Press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Niimi A, Suka N, Harata M, Kikuchi A, Mizuno S.: "Co-localization of chicken DNA topoisomerase IIalpha, but not beta, with sites of DNA replication and possible involvement of a C-terminal region of alpha through its binding to PCNA"Chromosoma. 110. 102-114 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yoshikawa,H.: "To open or not to open that is the question : replication of an entire chromosome."Cellular and Molecular Life Sciences. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] N.Sugata,S.Li,W.Earnshaw,T.Yen,K.Yoda,H.Masumoto,E.Munekata,P.Warburton and K.Todokoro:: "Human CENP-H multimers colocalize with CENP-A and CENP-C at active centromere-kinetochore complexes."Human Mol.Genet,. 9. 2919-2926 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Masahiro Nogami,Atsushi Kohda,M.Nakao,H.Taguchi,T.Ikemura,and K.Okumura,: "Relative Locations of the Centromere and Imprinted SNRPN Gene within Chromosome 15 Territories During the Cell Cycle in HL60 Cells"J.Cell Sci.. 113. 2157-2165 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tatsumi Y,Tsurimoto T,Shirahige K,Yoshikawa H,Obuse C.: "Association of human origin recognition complex 1 with chromatin DNA and nuclease-resistant nuclear structures."J.Biol.Chem.. 275. 5904-5910 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y.OKADA,Y.ITO,A.KIKUCHI,Y.NIMUR A,S.YOSHIDA and M.SUZUKI: "Assignment of functional aminoacids around the active site of human DNA topoisomerase llα""J.Biol.Chem.275,24630-24638(2000). 275. 24630-24638 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tatsumi Y.: "Association of human origin recognition complex 1 with chromatin DNA and nuclease-resistant nuclear structures"Journal of Biological Chemistry. 275(8). 5904-5910 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 吉川 寛: "複製制御とS期チェック制御の連結"実験医学. 17(4). 484-486 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Matsumoto K.: "Speru chromatin decondensation by template activating factor I through direct interaction with basic proteins"Molecular and Cellar Biology. 19. 6940-6952 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Haraguti T.: "Live fluoreseense imaging reveals early recruitment of emerin,LBR,RanBP2, and Nup153 to reforming functional nuclear envelopes"Journal of Cell Science. 113(P+5). 779-794 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Toya M.: "Fission yeast ,which is homologous to budding yeast Boi proteins and exhibits subcellular localization close to actin patches, is essential for cell elongation and separation"Molecular and Cellular Biology. 10(8). 2745-2757 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Maksum R.: "The cloning and characterization of CDCSO gene family in S.cerevisiae"Yeast. (予定).

    • Related Report
      1999 Annual Research Report

URL: 

Published: 1999-04-01   Modified: 2018-03-28  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi