Functions of RecQ family helicases in DNA replication, repair, and damage avoidance

• Project/Area Number: 17013005
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tohoku University
• Principal Investigator: ENOMOTO Takemi Tohoku University, 大学院・薬学研究科, 教授 (80107383)
• Project Period (FY): 2005 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥71,500,000 (Direct Cost: ¥71,500,000); Fiscal Year 2009: ¥14,300,000 (Direct Cost: ¥14,300,000); Fiscal Year 2008: ¥14,300,000 (Direct Cost: ¥14,300,000); Fiscal Year 2007: ¥14,300,000 (Direct Cost: ¥14,300,000); Fiscal Year 2006: ¥14,300,000 (Direct Cost: ¥14,300,000); Fiscal Year 2005: ¥14,300,000 (Direct Cost: ¥14,300,000)
• Keywords: 癌 / ブルーム症候群 / ロスモンド・トムソン症候群 / RecQファミリーへリカーゼ / DNA複製 / DNA組換え / 姉妹染色分体接着 / RecQファミリーヘレカーゼ / DNA傷害回避 / BLM / RECQL4 / KNA組換え / ブルーム症侯群 / RecQフミリーヘリカーゼ / RECL4 / RecQヘリカーゼ / Sgs1 / DNA複製フォーク / 傷害回避 / ワーナー症候群 / Sgsl / 障害回避

Research Abstract

Human cells possess five RecQ family helicases, RECQL1, BLM, WRN, RTS, and RECQL5, whose defects cause a predisposition to cancer. In this study, functions of these RecQ family helicases were investigated and following results were obtained. BLM functions at the last stage of DNA replication, segregation of replicated DNA and sister chromatid cohesion. RTS is involved in DNA replication and repair of DNA double strand breaks. Finally, RECQL1 and RECQL5 are involved in early stages of recombination.

Research Products

• [Journal Article] RecQL5 promotes genome stabilization trough two parallel mechanisms-Interacting with RNA polymerase II and acting as a helicase (2010)
  • Author(s): Islam, M.N., Fox. D. III, Guo, R., Enomoto, T., Wang, W
  • Journal Title: Mol. Cell. Biol 30 Pages: 2460-2472
  • Peer Reviewed
• [Journal Article] Deregulated Cdc6 inhibits DNA replication and suppresses Cdc7-dependent phosphorylation of Mcm2-7 complex in Xenopus egg extracts (2010)
  • Author(s): Kundu, L.R.
  • Journal Title: Nuc.Acids Res. (in press)
  • Peer Reviewed
• [Journal Article] RecQL5 promotes genome stabilization through two parallel mechanism Interacting with RNA polymerase II and acting as a helicase (2010)
  • Author(s): Islam, M. N.
  • Journal Title: Mol.Cell.Biol. (in press)
  • Peer Reviewed
• [Journal Article] Global analysis of mutual interaction surfaces of nucleosomes with comprehensive point mutants (2009)
  • Author(s): Sakamoto, M., Noguchi, S., Kawashima, S., Okada, Y., Enomoto, T., Seki, M., Horikoshi, M
  • Journal Title: Genes Cells 14 Pages: 1271-1330
  • Peer Reviewed
• [Journal Article] Structure of the Cdt1 C-terminal domain: conservation of the winged helix fold in replication licensing factors (2009)
  • Author(s): Khayrutdinov, B.I., Bae, W.J., Yun, Y.M., Lee, J.H., Tsuyama, T., Kim. J.J., Hwang, E., Ryu, K.S., Cheong, H.K., Cheong, C., Ko, J.S., Enomoto, T., Karplus, P.A., Guntert. P., Tada, S., Jeon, Y.H., Cho, Y
  • Journal Title: Protein Sci 18 Pages: 2252-2264
  • Peer Reviewed
• [Journal Article] Effect of heliquinomycin on the activity of human MCM4/6/7 helicase (2009)
  • Author(s): Ishimi, Y., Sugiyama, T., Nakaya, R., Kanamori, M., Kohno, T., Enomoto, T., Chino, M
  • Journal Title: FEBS J 276 Pages: 3382-3391
  • Peer Reviewed
• [Journal Article] Accumulation of sumoylated Rad52 in checkpoint mutants perturbed in DNA replication (2009)
  • Author(s): Ohuchi, T., Seki, M., Kugou, K., Tada, S., Ohta, K., Enomoto, T
  • Journal Title: DNA Repair 8 Pages: 690-696
  • Peer Reviewed
• [Journal Article] Alternative title: Generation and characterization of cells that can be conditionally depleted of mitochondrial SOD2 (2009)
  • Author(s): Takada, S., Inoue, E., Tano, K., Yoshii, H., Abe, T., Yoshimura, A., Akita, M., Tada, S., Watanabe, M., Seki, M., Enomoto, T
  • Journal Title: Biochem. Biophys. Res. Commun 379 Pages: 233-238
  • Peer Reviewed
• [Journal Article] Repression of nascent strand elongation by deregulated Cdt1 during DNA replication in Xenopus egg extracts (2009)
  • Author(s): Tsuyama, T., Watanabe, S., Aoki, A., Cho, Y., Seki, M., Enomoto, T., Tada, S
  • Journal Title: Mol. Bio. Cell 20 Pages: 937-947
  • Peer Reviewed
• [Journal Article] Global analysis of mutual interaction surfaces of nucleosomes with comprehensive point mutants (2009)
  • Author(s): Sakamoto, M.
  • Journal Title: Genes Cells 14 Pages: 1271-1330
  • Peer Reviewed
• [Journal Article] Structure of the Cdt1 C-terminal domain : conservation of the winged helix fold in replication licensing factors (2009)
  • Author(s): Khayrutdinov, B.
  • Journal Title: Protein Sci. 18 Pages: 2252-2264
  • Peer Reviewed
• [Journal Article] Effect of heliquinomycin on the activity of human MCM4/6/7 helicase (2009)
  • Author(s): Ishimi, Y.
  • Journal Title: FEBS Journal 276 Pages: 3382-3391
  • Peer Reviewed
• [Journal Article] Physical and functional interaction between WRNIPI and RAD18 (2009)
  • Author(s): Yoshimura, A.
  • Journal Title: Genes Genet.System 84 Pages: 171-178
  • Peer Reviewed
• [Journal Article] Accumulation of sumoylated Rad52 in checkpoint mutants perturbed in DNA replication (2009)
  • Author(s): Ohuchi, T.
  • Journal Title: DNA Repair 8 Pages: 690-696
  • Peer Reviewed
• [Journal Article] Generation and characterization of cells that can be conditionally depleted of mitochondrial SOD2 (2009)
  • Author(s): Takada, S.
  • Journal Title: Biochem.Biophys.Res.Commun. 379 Pages: 233-238
  • Peer Reviewed
• [Journal Article] Repression of nascent strand elongation by deregulated Cdt1 during DNA replication in Xenopus egg extracts (2009)
  • Author(s): Tsuyama, T.
  • Journal Title: Mol.Biol.Cell 20 Pages: 937-947
  • Peer Reviewed
• [Journal Article] Repression of nascent strand elongation by deregulated Cdtl during DNA replication in Xenonus egg extracts (2009)
  • Author(s): Tsuyama, T
  • Journal Title: Mol. Biol. Cell 20 Pages: 937-947
  • Peer Reviewed
• [Journal Article] Generation and characterization of cells that can be conditionally depleted of mitochondrial SOD2 (2009)
  • Author(s): Takada, S
  • Journal Title: Biochem. Biophys. Res. Commun 379 Pages: 233-238
  • Peer Reviewed
• [Journal Article] Accumnlatin of sumoylated Rad52 in checkpoint mutants perturbed in DNA replication (2009)
  • Author(s): Ohuchi, T
  • Journal Title: DNA Repair (in press)
  • Peer Reviewed
• [Journal Article] A novel function for Rad17 in homologous recombination (2008)
  • Author(s): Nishino, K., Inoue, E., Takada, S., Abe, T., Akita, M., Yoshimura, A., Tada, S., Kobayashi, M., Yamamoto, K., Enomoto, T
  • Journal Title: Genes Genet. Syst 83 Pages: 427-431
  • Peer Reviewed
• [Journal Article] RMI, a new OB-fold complex essential for Bloom syndrome protein to maintain genome stability (2008)
  • Author(s): Xu, D., Guo, R., Sobeck, A., Bachrati, C.Z., Yang, J., Enomoto, T., Brown, G.W., Hoatlin, M.E., Hickson, I.D., Wang W
  • Journal Title: Gene Dev 22 Pages: 2843-2855
  • Peer Reviewed
• [Journal Article] KU70/80, DNA-PKcs, and Artemis are essential for the rapid induction of apoptosis after massive DSB formation (2008)
  • Author(s): Abe, T., Ishiai, M., Hosono, Y., Yoshimura, A., Tsuji, H., Tada, S., Adachi, N., Koyama, H., Takata, M., Takeda, S., Enomoto, T., Seki, M
  • Journal Title: Cell Signal 20 Pages: 1978-1985
  • Peer Reviewed
• [Journal Article] Nuclear localization of Rad52 is pre-requisite for its sumoylation (2008)
  • Author(s): Ohuchi, T., Seki, M., Enomoto, T
  • Journal Title: Biochem. Biophys. Res. Commun 372 Pages: 126-130
  • Peer Reviewed
• [Journal Article] Rad52 sumoylation and its involvement in the efficient induction of homologous recombination (2008)
  • Author(s): Ohuchi, T., Seki, M., Branzei, D., Maeda, D., Ui, A., Ogiwara, H., Tada, S., Enomoto, T
  • Journal Title: DNA Repair 7 Pages: 879-889
  • Peer Reviewed
• [Journal Article] Analyses of functional interaction between RECQL1, RECQL5, and BLM which physically interact with DNA topoisomerase III・ (2008)
  • Author(s): Otsuki, M., Seki, M., Inoue, E., Abe. T., Narita, Y., Yoshimura, A., Tada, S., Ishii, Y., Enomoto, T
  • Journal Title: Biochim. Biophys. Acta 1782 Pages: 75-8
  • Peer Reviewed
• [Journal Article] Analyses of functional interaction between RECQL1。 RECQL5, and BLM which physically interact with DNA tonoisomerase IIIα (2008)
  • Author(s): Otsuld, M
  • Journal Title: Biochim. Biophys. Acta 1782 Pages: 75-81
  • Peer Reviewed
• [Journal Article] Rad52 sumoylation and its involvement in the efficient induction of homologous recombination (2008)
  • Author(s): Ohuchi, T
  • Journal Title: DNA Repair 7 Pages: 879-889
  • Peer Reviewed
• [Journal Article] Nuclear localization of Rad52 is pre-requisite for its sumoylation (2008)
  • Author(s): Ohuchi, T
  • Journal Title: Biochem. Biophys. Res. Commun 372 Pages: 126-130
  • Peer Reviewed
• [Journal Article] RMI, a new OB-fold complex essential for Bloom syndrome protein to maintain genome stability (2008)
  • Author(s): Xu, D
  • Journal Title: Genes Dev 22 Pages: 2843-2855
  • Peer Reviewed
• [Journal Article] A novel function for Rad17 in homologous recombination (2008)
  • Author(s): Nishino, K
  • Journal Title: Genes Genet. System 83 Pages: 427-431
  • Peer Reviewed
• [Journal Article] Rad52 sumoylation and its involvement in the efficient induction of homologous recombination (2008)
  • Author(s): Takashi Ohuchi
  • Journal Title: DNA Repair (In press)
  • Peer Reviewed
• [Journal Article] Vertebrate WRNIP1 and BLM are required for efficient maintenance of genome stability (2008)
  • Author(s): Tomoko Hayashi
  • Journal Title: Genes Genet. Syst. 83 Pages: 95-100
  • Peer Reviewed
• [Journal Article] Analyses of functional interaction between RECQL1, RECQL5, and BLM which physically interact with DNA topoisomerase IIIα (2008)
  • Author(s): Makoto Otsuki
  • Journal Title: Biochim. Biophys. Acta 1782 Pages: 75-81
  • Peer Reviewed
• [Journal Article] Functional interactions between BLM and XRCC3 in the cell (2007)
  • Author(s): Otsuki, M., Seki, M., Inoue, E., Yoshimura, A., Kato, G., Yamanouchi, S., Kawabe, Y., Tada, S., Shinohara, A., Komura, J., Ono, T., Takeda, S., Ishii, Y., Enomoto, T
  • Journal Title: J. Cell Biol 179 Pages: 53-63
  • Peer Reviewed
• [Journal Article] Ctf18 is required for homologous recombination -mediated double strand break repair (2007)
  • Author(s): Ogiwara, H., Ohuchi, T., Ui, A., Tada, S., Enomoto, T., Seki, M
  • Journal Title: Nuc. Acids Res 35 Pages: 4989-5000
  • Peer Reviewed
• [Journal Article] Rmi1, a member of the Sgs1-Top3 complex in budding yeast, contributes to sister chromatid cohesion (2007)
  • Author(s): Lai M.S., Seki, M., Ui, A., Enomoto, T
  • Journal Title: EMBO Reports 8 Pages: 685-690
  • Peer Reviewed
• [Journal Article] Activation of a novel pathway involving Mms1 and Rad59 in sgs1 cells (2007)
  • Author(s): Ui, A., Seki, M., Ogiwara, H., Lai, M.S., Yamamoto, K., Tada, S., Enomoto, T
  • Journal Title: Biochem. Biophys. Res. Commun 356 Pages: 1031-1037
  • Peer Reviewed
• [Journal Article] INO80 chromatin remodeling complex functions in sister chromatid cohesion (2007)
  • Author(s): Ogiwara, H., Enomoto, T., Seki, M
  • Journal Title: Cell Cycle 6 Pages: 1090-1095
  • Peer Reviewed
• [Journal Article] Actin-related protein Arp4 functions in kinetochore construction (2007)
  • Author(s): Ogiwara, H., Ui, A., Kawashima, S., Kugou, K., Onoda, F., Iwahashi, H., Harata, M., Ohta, K., Enomoto, T., Seki, M
  • Journal Title: Nuc. Acids Res 35 Pages: 3109-3117
  • Peer Reviewed
• [Journal Article] INO80 complex is required for damage-induced recombination (2007)
  • Author(s): Kawashima, S., Ogiwara, H., Tada, S., Harata, M., Wintersberger, U., Enomoto, T., Seki, M
  • Journal Title: Biochem. Biophys. Res. Commun 355 Pages: 835-841
  • Peer Reviewed
• [Journal Article] Ames test-negative carcinogen, ortho-phenyl phenol, binds tubulin and causes aneuploidy in budding yeast (2007)
  • Author(s): Nunoshiba, T., Watanabe, E., Takahashi, T., Daigaku., Y., Ishikawa, S., Mochizuki, M., Ui, A., Enomoto, T., Yamamoto, K
  • Journal Title: Mut. Res 617 Pages: 90-97
  • Peer Reviewed
• [Journal Article] Rad50 is involved in MMS-induced recombination between homologous chromosomes in mitotic cells (2007)
  • Author(s): Tomizawa, Y., Ui, A., Onoda, F., Ogiwara, H., Tada, S., Enomoto, T., Seki, M
  • Journal Title: Genes Genet. Syst 82 Pages: 157-160
  • NAID: 10024394124
  • Peer Reviewed
• [Journal Article] Possible involvement of RecQL4 in the repair of double-strand DNA breaks in Xenopus egg extracts (2007)
  • Author(s): Kumata, Y., Tada, S., Yamanada, Y., Tsuyama, T., Kobayashi, T., Dong, Y. -P., Ikegami, K., Murofushi, H., Seki, M., Enomoto, T
  • Journal Title: Biochim. Biophys. Acta 1773 Pages: 556-564
  • Peer Reviewed
• [Journal Article] Chl1 and Ctf4 are required for damage-induced recombinations. Biochem (2007)
  • Author(s): Ogiwara, H., Ui, A., Lai M.S., Enomoto, T., Seki M
  • Journal Title: Biophys. Res. Commun 354 Pages: 222-226
  • Peer Reviewed
• [Journal Article] Role of Elg1 protein in the double-strand breaks repair (2007)
  • Author(s): Ogiwara, H., Ui, A., Enomoto, T., Seki, M
  • Journal Title: Nuc. Acids Res 35 Pages: 353-362
  • Peer Reviewed
• [Journal Article] Functional interactions between BLM and XRCC3 in the cell (2007)
  • Author(s): Makoto Otsuki
  • Journal Title: J. Cell Biol. 179 Pages: 53-63
  • Peer Reviewed
• [Journal Article] Ctfl8 is required for homologous recombination-mediated. double-strand break repair (2007)
  • Author(s): Hideaki Ogiwara
  • Journal Title: Nucleic Acids Res. 35 Pages: 4989-5000
  • Peer Reviewed
• [Journal Article] Rmil, a member of the Sgsl-Top3 complex in budding yeast, contributes to sister chromatidcohesion (2007)
  • Author(s): Mong Sing Lai
  • Journal Title: EMBO Rep. 8 Pages: 685-690
  • Peer Reviewed
• [Journal Article] Activation of a novel pathway involving Mmsl and Rad59 in sgs1 cells (2007)
  • Author(s): Ayako Ui
  • Journal Title: Biochem. Biophys. Res. Commun. 356 Pages: 1031-1037
  • Peer Reviewed
• [Journal Article] The INO80 complex is required for damage-induced recombination (2007)
  • Author(s): Satoshi Kawashima
  • Journal Title: Biochem. Biophys. Res. Commun. 355 Pages: 835-841
  • Peer Reviewed
• [Journal Article] Possible involvement of RecQL4 in the repair of double-strand DNA breaks in Xenopus egg extracts (2007)
  • Author(s): Yuji Kumata
  • Journal Title: Biochim. Biophys. Acta 1773 Pages: 556-564
  • Peer Reviewed
• [Journal Article] The INO80 chromatin remodeling complex functions in sister chromatid cohesion (2007)
  • Author(s): Hideaki Ogiwara
  • Journal Title: Cell Cycle 6 Pages: 1090-1095
  • Peer Reviewed
• [Journal Article] Actin-related protein Arp4 functions in kinetochore assembly (2007)
  • Author(s): Hideaki Ogiwara
  • Journal Title: Nucleic Acids Res. 35 Pages: 3109-3117
  • Peer Reviewed
• [Journal Article] Rad50 is involved in MMS-induced recombination between homologous chromosomes in mitotic cells. (2007)
  • Author(s): Yuji Tomizawa
  • Journal Title: Genes Genet. Syst. 82 Pages: 157-160
  • Peer Reviewed
• [Journal Article] Chll and Ctf4 are required for damage-induced recombinations (2007)
  • Author(s): Hideaki Ogiwara
  • Journal Title: Biochem. Biophys. Res. Commun. 354 Pages: 222-226
  • Peer Reviewed
• [Journal Article] Role of Elg1 protein in the double-strand breaks repair (2007)
  • Author(s): Hideaki Ogiwara
  • Journal Title: Nucleic Acids Res. 35 Pages: 353-362
  • Peer Reviewed
• [Journal Article] Role of Elg1 protein in the double-strand breaks repair. (2007)
  • Author(s): Hideaki Ogiwara
  • Journal Title: Nucleic Acids Res. 35・2 Pages: 353-362
• [Journal Article] Chll and Ctf4 are required for damage-induced recombinations. (2007)
  • Author(s): Hideaki Ogiwara
  • Journal Title: Biochem. Biophys. Res. Commun. 354・1 Pages: 222-226
• [Journal Article] RecQL4 functions in Ku and DNA-dependent protein kinase-mediated double-strand break repair in Xenopus egg extracts. (2007)
  • Author(s): Yuji Kumata
  • Journal Title: Biochim. Biophys. Acta 1773・4 Pages: 556-564
• [Journal Article] INO80 complex is required for damage-induced recombination. (2007)
  • Author(s): Satoshi Kawashima
  • Journal Title: Biochem. Biophys. Res. Commun. 355・3 Pages: 835-841
• [Journal Article] Actin-related protein Arp4 functions in kinetochore construction. (2007)
  • Author(s): Hideaki Ogiwara
  • Journal Title: Nucleic Acids Res. (in press)
• [Journal Article] INO80 chromatin remodeling complex functions in sister chromatid cohesion. (2007)
  • Author(s): Hideaki Ogaiwara
  • Journal Title: Cell Cycle (in press)
• [Journal Article] Chromatin loading of Smc5/6 is induced by DNA replication but not by DNA double-strand breaks (2006)
  • Author(s): Tsuyama, T., Inou, K., Seki, M., Seki, T., Kumata, Y., Kobayashi, T., Kimura, K., Hanaoka, F., Enomoto, T., Tada, S
  • Journal Title: Biochem. Biophys. Res. Commun 351 Pages: 935-939
  • Peer Reviewed
• [Journal Article] Mgs1 and Rad18/Rad5/Mms2 are required for survival of novel temperature/cold sensitive mutant alleles of Pol31, the second subunit of DNA polymerase・, in Saccharomyces cerevisiae (2006)
  • Author(s): Davoodi Vijeh Motlagh, N., Branzei, D., Seki, M., Enomoto, T
  • Journal Title: DNA Repair 5 Pages: 1459-1474
  • Peer Reviewed
• [Journal Article] A novel Rad18 function involved in the protection of the vertebrate genome after exposure to camptothecin (2006)
  • Author(s): Yoshimura, A., Nishino, K., Takezawa, J., Tada, S., Kobayashi, T., Sonoda, E., Kawamoto, T., Takeda, S., Ishii, Y., Yamada, K., Enomoto, T., Seki, M
  • Journal Title: DNA Repair 5 Pages: 1307-1316
  • Peer Reviewed
• [Journal Article] Ubc9 and Mms21 mediated sumoylation counteracts recombinogenic events at damaged replication forks (2006)
  • Author(s): Branzei, D., Sollier, J., Liberi, G., Zhao, X, Maeda, D., Seki, M., Enomoto, T., Ohta, K., Foiani, M
  • Journal Title: Cell 127 Pages: 509-522
  • Peer Reviewed
• [Journal Article] BLM is an early responder to DNA double-strand breaks. Biochem. Biophys (2006)
  • Author(s): Karmakar, P., Seki, M., Kanamori, M., Hashiguchi, K., Ohtsuki, M., Murata, E., Inoue, E., Tada, S., Lan, L., Yasui, A., Enomoto, T
  • Journal Title: Res. Commun 348 Pages: 62-69
  • Peer Reviewed
• [Journal Article] Loss of heterozygosity in yeast can occur by ultraviolet irradiation during the S phase of the cell cycle (2006)
  • Author(s): Daigaku, Y., Mashiko, S., Mishiba, K., Yamamura, S., Ui, A., Enomoto, T., Yamamoto, K
  • Journal Title: Mutat. Res 600 Pages: 177-183
  • Peer Reviewed
• [Journal Article] Bloom helicase and DNA topoisomerase III・are involved in the dissolution of sister chromatids (2006)
  • Author(s): Seki, M., Nakagawa, T., Seki, T., Kato, G., Tada, T., Takahashi, Y., Yoshimura, A., Kobayashi, T., Aoki, A., Otsuki, M., Habermann, F.A., Tanabe, H., Ishii, Y., Enomoto, T
  • Journal Title: Mol. Cell. Biol 26 Pages: 6299-6307
  • Peer Reviewed
• [Journal Article] Dpb11, the budding yeast homologue of TopBP1, functions with the checkpoint clamp in recombination repair (2006)
  • Author(s): Ogiwara, H., Ui, A., Onoda, F., Tada, S., Enomoto, T., Seki, M
  • Journal Title: Nuc. Acids Res 34 Pages: 3389-3398
  • Peer Reviewed
• [Journal Article] Ubc9 and Mms21 mediated sumoylation counteracts recombinogenic events at demaged replication forks. (2006)
  • Author(s): Dana Branzei
  • Journal Title: Cell 127・3 Pages: 509-522
• [Journal Article] Bloom helicase and DNA topoisomerase IIIα are involved in the dissolution of sister chromatids. (2006)
  • Author(s): Masayuki Seki
  • Journal Title: Mol. Cell. Biol. 26・16 Pages: 6299-6307
• [Journal Article] Analyses of the interaction of WRNIP1 with Werner syndrome protein (WRN) in vitro and in the cell. (2006)
  • Author(s): Yoh-ichi Kawabe
  • Journal Title: DNA Repair 5・7 Pages: 816-828
• [Journal Article] Dpb11, the budding yeast homologue of TopBP1, functions with the checkpoint clamp in recombination repair. (2006)
  • Author(s): Hideaki Ogiwara
  • Journal Title: Nucleic Acids Res. 34・11 Pages: 3389-3398
• [Journal Article] BLM is an early responder to DNA double-strand breaks. (2006)
  • Author(s): Parimal Karmakar
  • Journal Title: Biochem. Biophys. Res. Commun. 348・1 Pages: 62-69
• [Journal Article] A novel Rad18 function involved in the protection of the vertebrate genome after exposure to camptothecin. (2006)
  • Author(s): Akari Yoshimura
  • Journal Title: DNA Repair 5・11 Pages: 1307-1316
• [Journal Article] Mgsl and Rad18/Rad5/Mms2 are require for survival of novel temperature/cold sensitive mutant alleles of Pol31, the second subunit of DNA polymerase d, in Sacharomyces cerevisiae. (2006)
  • Author(s): N.Davoodi Vijeh Motlagh
  • Journal Title: DNA Repair 5・12 Pages: 1459-1474
• [Journal Article] Chromatin loading of Smc5/6 is induced by DNA replication but not by DNA double-strand breaks. (2006)
  • Author(s): Takashi Tsuyama
  • Journal Title: Biochem. Biophys. Res. Commun. 351・4 Pages: 935-939
• [Journal Article] Caenorhabditis elegans geminin homologue participates in cell cycle regulation and germ line development (2005)
  • Author(s): Yanagi, K., Mizuno, T., Tsuyama, T., Tada, S., Iida, Y., Sugimoto, A., Eki, T., Enomoto, T., Hanaoka F
  • Journal Title: J. Biol. Chem 280 Pages: 19689-19694
  • Peer Reviewed
• [Journal Article] Functional relationships of FANCC to homologous recombination, translesion synthesis, and BLM (2005)
  • Author(s): Hirano, S., Yamamoto, K., Ishiai, M., Yamazoe, M., Seki, M., Matsushita, N., Ohzeki, M., Yamashita, Y.M., Arakawa, H., Buerstedde, J. -M., Enomoto, T., Takeda, S., Thompson, L.H., Takata, M
  • Journal Title: EMBO J 24 Pages: 418-427
  • Peer Reviewed
• [Journal Article] Human Werner helicase interacting protein 1 (WRNIP1) functions as a novel modulator for DNA polymerase・・ (2005)
  • Author(s): Tsurimoto, T., Shinozaki, A., Yano, M., Seki, M., Enomoto, T
  • Journal Title: Genes Cells 10 Pages: 13-22
  • Peer Reviewed
• [Journal Article] DNA topoisomerase III is essential for damage-induced recombination (2005)
  • Author(s): Ui, A., Seki, M., Ogiwara, H., Onodera, R., Fukushige, S., Onoda, F., Enomoto, T
  • Journal Title: DNA Repair 4 Pages: 191-201
  • Peer Reviewed
• [Journal Article] The ability of Sgs1 to interact with DNA topoisomerase III is essential for damage-induced recombination. (2005)
  • Author(s): Ayako Ui
  • Journal Title: DNA Repair 4 Pages: 191-201
• [Journal Article] Epistasis between Fanconi anemia protein FANCC and BLM helicase in suppressing sister chromatid exchanges. (2005)
  • Author(s): Seiki Hirano
  • Journal Title: EMBO Journal 24 Pages: 418-427
• [Journal Article] Licensing for DNA replication requires a strict sequential assembly of Cdc6 and Cdt1 onto chromatin in Xenopus egg extracts. (2005)
  • Author(s): Takashi Tsuyama
  • Journal Title: Nucleic Acids Research 33 Pages: 765-775
• [Journal Article] Caenorhabditis elegans eminin homologue participates in cell cycle regulation and germ line development. (2005)
  • Author(s): Ken-ichiro Yanagi
  • Journal Title: J.Biol.Chem. 280 Pages: 19689-19694
• [Journal Article] Human Werner helicase interacting protein 1 (WRNIP1) functions as a novel modulator for DNA polymerase δ. (2005)
  • Author(s): Toshiki Tsurimoto
  • Journal Title: Genes to Cells 10 Pages: 12-22
• [Presentation] Acetylation of histone H3-K56 regulates homologous recombination (2009)
  • Author(s): 遠藤博人
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2009-12-11
• [Presentation] A mutant protein of histone chaperone CIA/Asf1 defective in disrupting histone(H3-H4)_2 tetramer accelerates rate of DNA replication (2009)
  • Author(s): 大隅達也
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2009-12-11
• [Presentation] Analysis of DNA replication repression by excess Cdt1 (2009)
  • Author(s): 牛田磨理
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2009-12-10
• [Presentation] Nuclear superoxide may trigger spontaneous sister chromatid exchanges (2009)
  • Author(s): 井上絵里
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2009-12-09
• [Presentation] 親鎖ヌクレオソーム中のヒストン成分の娘鎖への分配機構解明に向けて (2009)
  • Author(s): 石川勝幸
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: ポートアイランド(神戸)
  • Year and Date: 2009-10-23
• [Presentation] DNA複製チェックポイント因子 Claspin とRad17の機能的関連の解析 (2009)
  • Author(s): 細野嘉史
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: ポートアイランド(神戸)
  • Year and Date: 2009-10-23
• [Presentation] DNA複製に必須なCdc45の新規機能の解析 (2009)
  • Author(s): 大隅達也
  • Organizer: 第48回日本薬学会東北支部大会
  • Place of Presentation: 東北薬科大学(仙台)
  • Year and Date: 2009-10-18
• [Presentation] ヒストンシャペロンFACTのDNA複製における機能解析 (2009)
  • Author(s): 阿部拓也
  • Organizer: 第6回 東北大学バイオサイエンスシンポジウム
  • Place of Presentation: 仙台国際センター(仙台)
  • Year and Date: 2009-06-16
• [Presentation] 染色体分配におけるヒストンの機能的役割とクロマチン制御機構の解析 (2009)
  • Author(s): 川嶋聡
  • Organizer: 第6回 東北大学バイオサイエンスシンポジウム
  • Place of Presentation: 仙台国際センター(仙台)
  • Year and Date: 2009-06-16
• [Presentation] Regulation of DNA replication initiation by Cdc6 and its implication in anti-cancer drug desining (2009)
  • Author(s): Kundu, L.R.
  • Organizer: 第6回 東北大学バイオサイエンスシンポジウム
  • Place of Presentation: 仙台国際センター(仙台)
  • Year and Date: 2009-06-16
• [Presentation] The role of FACT in DNA replication (2009)
  • Author(s): Takemi Enomoto
  • Organizer: Swiss-Japan joint metting on the molecular mechanisms regulating "Chromosome dynamics and Genome stability"
  • Place of Presentation: Villars Ollons, Switzerland
  • Year and Date: 2009-05-15
• [Presentation] The role of FACT in DNA replication (2009)
  • Author(s): Enomoto, T.
  • Organizer: Swiss-Japan joint meeting on the molecular mechanisms regulating "Chromosome dynamics and genome stability"
  • Place of Presentation: Villars Ollons(スイス)
  • Year and Date: 2009-05-15
• [Presentation] DNA傷害に応じたRecQL4の動態と相互作用タンパク質の機能解明 (2008)
  • Author(s): 池上京子
  • Organizer: 第31回日本分子生物学会年会第81回日本生化学会大会合同大会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2008-12-12
• [Presentation] 高等動物細胞におけるチェックポイント因子Claspinの機能の解析 (2008)
  • Author(s): 秋田玄武
  • Organizer: 第31回日本分子生物学会年会第81回日本生化学会大会合同大会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2008-12-12
• [Presentation] ブルーム症候群原因遺伝子産物BLMのDNA複製及び姉妹染色分体接着における役割(Functions of the Bloom syndrome responsible gene product in DNA replication and sister chromatid cohesion) (2008)
  • Author(s): 榎本武美
  • Organizer: 日本環境変異原学会第37回大会
  • Place of Presentation: 沖縄
  • Year and Date: 2008-12-05
• [Presentation] ブルーム症候群原因遺伝子産物BLMのDNA複製及び姉妹染色分体接着における役割 (2008)
  • Author(s): 榎本武美
  • Organizer: 日本環境変異原学会第37回大会
  • Place of Presentation: 沖縄県宜野湾
  • Year and Date: 2008-12-05
• [Presentation] Effects of deregulating Cdc6 during the initiation of DNA replication (2008)
  • Author(s): Kundu, R. L
  • Organizer: The 6th 3R Symposium
  • Place of Presentation: 静岡県嬬恋市
  • Year and Date: 2008-10-27
• [Presentation] Functional analysis of RecQL4 using RecQL4 mutated cells (2008)
  • Author(s): Abe, T
  • Organizer: The 6th 3R Symposium
  • Place of Presentation: 静岡県嬬恋市
  • Year and Date: 2008-10-27
• [Presentation] Rmi1, a member of the Sgs1-Top3 complex in budding yeast, contributes to sister chromatid cohesion (2008)
  • Author(s): Mong Sing Lai, Masayuki Seki, Ayako Ui, Takemi Enomoto
  • Organizer: 2008 EMBO conference on DNA recombination
  • Place of Presentation: Cioccol, Italy
  • Year and Date: 2008-05-21
• [Presentation] Rmil, a member of the Sgsl-Top3 complex in budding yeast, contributes to sister chromatid cohesion (2008)
  • Author(s): Lai, M. S
  • Organizer: 2008 EMBO conference on DNA recombination
  • Place of Presentation: I1 Cioccol, Italy
  • Year and Date: 2008-05-21
• [Presentation] RecQL4のDNA二本鎖切断誘発時における挙動の解析 (2008)
  • Author(s): 池上京子、多田周右、Li Lan、安井明、関政幸、榎本武美
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 横浜
  • Year and Date: 2008-03-27
• [Presentation] Illegitimate activation of Cdt1 at S-phase possibly leads to a halt of nascent strand elongation in DNA re-replication (2007)
  • Author(s): Shusuke Tada, Masayuki Seki, Takemi Enomoto
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-03
• [Presentation] Novel functions of Sgsl (BLM), Top3 (TopoIII alpha), and Rmi1 (BLAP75) during DNA replication (2007)
  • Author(s): Mong Sing Lai
  • Organizer: 第3回日米DNA修復会議
  • Place of Presentation: 仙台市
  • Year and Date: 2007-05-07
• [Presentation] BLM functions together with XRCC3 for resumption of stalled replication forks (2006)
  • Author(s): Makoto Otsuki, Masayuki Seki, Eri Inoue, Genta Katoh, Akari Yoshimura, Shusuke Tada, Yutaka Ishii, Shunichi Takeda, Takemi Enomoto
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB congress
  • Place of Presentation: Kyoto
  • Year and Date: 2006-06-18
• [Presentation] Rmi1/Nce4, a subunit of the Sgs1-Top3 complex of Saccharomyces cerevisiae is involved in homologous recombination and establishment of sister chromatid cohesion (2006)
  • Author(s): Mong Sing Lai、宇井彩子、関政幸、榎本武美
  • Organizer: 日本薬学会第126年会
  • Place of Presentation: 仙台
  • Year and Date: 2006-03-29
• [Presentation] DNA二本鎖切断修復におけるRecQL4の機能の解析 (2006)
  • Author(s): 山名田弓枝、多田周右、二藤望、熊田裕司、小林貴之、関政幸、榎本武美
  • Organizer: 日本薬学会第126年会
  • Place of Presentation: 仙台
  • Year and Date: 2006-03-28
• [Presentation] BLM is an early responder to DNA double strand breaks (2005)
  • Author(s): Parimal Karmakar, Masayuki Seki, Makoto Kanamori, Shusuke Tada, Li Lan, Kazunari Hashiguchi, Akira Yasui, Takemi Enomoto
  • Organizer: The 5th Symposium on DNA Replication, Recombination and Repair
  • Place of Presentation: Hyogo
  • Year and Date: 2005-11-13
• [Presentation] BLM acts together with XRCC3 upon damage induction (2005)
  • Author(s): Makoto Otsuki, Masayuki Seki, Genta Kato, Eri Inoue, Akari Yoshimura, Shusuke Tada, Takemi Enomoto
  • Organizer: 第78回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2005-10-22
• [Presentation] ブルーム症候群原因遺伝子産物の機能の解析 (2005)
  • Author(s): 関政幸、多田周右、榎本武美
  • Organizer: 第64回日本癌学会学術総会
  • Place of Presentation: 札幌
  • Year and Date: 2005-09-14
• [Book] 蛋白質核酸酵素別冊;染色体サイクル (2009)
  • Author(s): 関政幸・Mong Sing Lai・榎本武美
  • Publisher: 共立出版
• [Book] 染色体サイクル (2009)
  • Author(s): 関政幸
  • Publisher: 共立出版
• [Book] Reviews and protocols in DT40 research (2006)
  • Author(s): Seki, M., Tada, S., Enomoto, T
  • Publisher: Springer
• [Book] DT40 Handbook (2006)
  • Author(s): Masayuki Seki
  • Total Pages: 477
  • Publisher: Springer
• [Book] DT40 Handbook (2006)
  • Author(s): Masayuki Seki
  • Publisher: Springer(In press)